Microarrays-Enabled Hypothesis Generation: The Suspect Role of FNBP-1 in Neuropsychiatric Pathogenesis Associated with HIV and/or HCV Infection
| Autor: | Antonios Katsounas, Richard A. Lempicki, Wilting Kr, Guido Gerken, Jörg F. Schlaak |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Microarray Hepatitis C virus medicine.medical_treatment Immunology Dermatology medicine.disease_cause Article Pathogenesis 03 medical and health sciences Interferon Virology medicine Neuropsychiatric disease Microarray analysis techniques business.industry HIV Immunotherapy Omics 030104 developmental biology Infectious Diseases HCV FNBP-1 DNA microarray business medicine.drug |
| Zdroj: | Journal of AIDS & clinical research |
| ISSN: | 2155-6113 |
| Popis: | Objective: The spectrum of neuropsychiatric illness (NI) associated with the Human Immunodeficiency Virus (HIV) and/or the Hepatitis C Virus (HCV) is far reaching and significantly impacts the clinical presentation and outcome of infected persons; however, the etiological and pathophysiological background remains partially understood. The present work was aimed to investigate the potential significance of formin binding protein 1 (FNBP-1)-dependent pathways in NI-pathogenesis by elaborating on previous microarray-based research in HIV and/or HCV-infected patients receiving interferon-? (IFN-?) immunotherapy via a rigorous data mining procedure. Methods: Using microarray data of peripheral whole blood (PB) samples obtained from HCV mono-infected persons (n=25, Affymetrix? HG-U133A_2) 12 h before and after the 1st dose of pegylated IFN-? (PegIFN-?), we reapplied the same analytical algorithm that we had developed and published in an earlier study with HIV/HCV coinfected subjects (N=28, Affymetrix? HG-U133A), in order to evaluate reproducibility of potential NI-related molecular findings in an independent cohort. Results: Among 28 gene expression profiles (HIV/HCV: N=9 vs. HCV: N=19) selected by applying different thresholds (a Mean Fold Difference value (MFD) in gene expression of ? 0.38 (log2) and/or P value from |
| Databáze: | OpenAIRE |
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