The effect of biguanides on secretion and biosynthesis of insulin in isolated pancreatic islets of rats
| Autor: | E. E. Pfeiffer, H Schatz, N. Katsilambros, C. Nierle |
|---|---|
| Rok vydání: | 1972 |
| Předmět: |
Male
medicine.medical_specialty medicine.drug_class Endocrinology Diabetes and Metabolism medicine.medical_treatment Biguanides In Vitro Techniques Phenformin Biology Tritium Glibenclamide Islets of Langerhans chemistry.chemical_compound Oxygen Consumption Leucine Internal medicine Glyburide Insulin Secretion Internal Medicine medicine Animals Insulin Drug Interactions Proinsulin Biguanide Pancreatic islets Rats Inbred Strains Metformin Rats Insulin oscillation Glucose Endocrinology medicine.anatomical_structure chemistry medicine.drug |
| Zdroj: | Diabetologia. 8:402-407 |
| ISSN: | 1432-0428 0012-186X |
| Popis: | Based on the clinical observation that biguanide treatment of obese patients may alter insulin levels, the influence of metformin and phenformin on basal and glucose stimulated insulin secretion, as well as on insulin biosynthesis, was studied in isolated islets of rats. — Biguanide concentrations of 100 μg/ml, or higher, significantly reduced glucose stimulated insulin secretion. Both dose dependence and a difference in the intrinsic activities of metformin and phenformin were demonstrated. Incubating the same islets for a second period without biguanides, glucose stimulated insulin secretion was still decreased. Addition of glibenclamide during this second period increased insulin secretion, but did not overcome complete inhibition achieved after incubation at very high biguanide concentrations. Glucose stimulated biosynthesis of proinsulin and insulin was decreased in the presence of biguanides and completely suppressed at very high concentrations. Inhibition of cell respiration in the islet cells effected by high biguanide doses may be the reason for the inhibition of secretion and biosynthesis of insulin. — On the other hand, an insulin release was found at the highest phenformin concentration of 10 mg/ ml and during perfusion of the isolated rat pancreas with higher biguanide doses. — Biguanide concentrations found to be effective in this study are very high compared with therapeutic levels. Moreover, biguanide actions are known to be highly dependent on species, concentration and metabolic situation. — Definite conclusions from these findings regarding clinical significance, therefore, seem unwarranted. |
| Databáze: | OpenAIRE |
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