ANALYSIS OF THE KINETICS OF GRANULOSA CELL POPULATIONS IN THE MOUSE OVARY
Autor: | N. R. Hartmann, Torben Pedersen |
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Rok vydání: | 1970 |
Předmět: |
medicine.medical_specialty
Time Factors Granulosa cell Kinetics Mitosis Probability density function Biology Tritium Standard deviation Normal distribution Mice Follicle Cell Movement Internal medicine Methods medicine Animals Doubling time Computers Ovary Rats Inbred Strains Cell Biology General Medicine Rats Endocrinology Biophysics Autoradiography Female Mathematics Thymidine |
Zdroj: | Cell Proliferation. 3:1-11 |
ISSN: | 1365-2184 0960-7722 |
DOI: | 10.1111/j.1365-2184.1970.tb00246.x |
Popis: | The kinetics of granulosa cell populations in two types of follicles in ovaries of 28-day-old Bagg mice are investigated. The analysis includes estimations of mean values and standard deviations of the transit times (TG1, TS, TG2 and TC), the doubling time TD, and the proliferative fraction p. First the percentage of labelled mitosis curve (PLM-curve) and the continuous labelling curve (CL-curve) are estimated. Then a hypothesis concerning the cell kinetics of the granulosa cells in the two follicle types is set up. The normal distribution is chosen to simulate the probability density functions of the transit times. On the basis of the hypothesis mathematical expressions for the PLM- and CL-curves are worked out. By fitting the calculated PLM-curve to the experimental one it is possible to estimate mean values and standard deviations of TG1, TS>, and TG2. As a test of the hypothesis the CL-curve is calculated by means of the estimated parameter values and compared to the experimental one. The calculated PLM- and CL-curves are found to be in good agreement with the experimental data as far as both follicle types are concerned. It is concluded that the method is a useful procedure. The choice of a normal distribution does not imply a significant limitation of the method in these investigations. Moreover it is concluded that the hypothesis is plausible. This means, e.g., that the proliferative fraction is unity in the two follicle types and that there is no cell loss from the cell systems. |
Databáze: | OpenAIRE |
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