Evaluation of intracellular and extracellular trehalose as a cryoprotectant of stem cells obtained from umbilical cord blood
| Autor: | Flávio Henrique Paraguassu-Braga, Luis Fernando Bouzas, Juliana Motta, Luís Cristóvão Porto |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Cryoprotectant
Cell Survival CD34 Biology General Biochemistry Genetics and Molecular Biology Cryopreservation chemistry.chemical_compound Cryoprotective Agents Humans Viability assay Cells Cultured Dimethyl sulfoxide Trehalose General Medicine Fetal Blood Hematopoietic Stem Cells Cell biology chemistry Biochemistry Cord blood Liposomes Female Stem cell General Agricultural and Biological Sciences |
| Zdroj: | Cryobiology. 68(3) |
| ISSN: | 1090-2392 |
| Popis: | Cord blood is a source of hematopoietic stem cells used in transplantation in which hematopoietic reconstitution is necessary. This transplant modality requires the cryopreservation of hematopoietic stem cells (HSCs). Dimethyl sulfoxide has been used as a cryoprotectant (CPA) in the cryopreservation of HSCs; however, it has been demonstrated that Me2SO exhibits toxic side effects to the human body. Due to its stability upon freezing, disaccharides such as trehalose have been investigated as a cryoprotectant. This study investigated the hypothesis that a cryopreservation solution containing intracellular and extracellular trehalose improves the recovery of stem cells after cryopreservation. After thawing, the cells were tested for their viability using the 7AAD stain, CD45+/CD34+ cells were assessed using flow cytometry and the MTT viability assay, and the proportion of hematopoietic progenitor cells was measured using the CFU assay. Our results showed the effectiveness of the solution containing intracellular and extracellular trehalose in the cryopreservation of cord blood cells, demonstrating that trehalose may be an optimal cryoprotectant when present both inside and outside of cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect