Interference with lncRNA NEAT1 promotes the proliferation, migration, and invasion of trophoblasts by upregulating miR-411-5p and inhibiting PTEN expression
| Autor: | Min Hu, Xiujuan Zheng, Ying Wang, Jiayue Wang, Xufei Fan, Jianyi Lou, Mengmeng Luo |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Immunology Toxicology Preeclampsia Cell Line 03 medical and health sciences 0302 clinical medicine Cell Movement Pregnancy medicine Immunology and Allergy PTEN Humans Cell Proliferation Pharmacology Reporter gene biology Cell growth Chemistry PTEN Phosphohydrolase RNA Trophoblast Gene Expression Regulation Developmental Cell migration General Medicine medicine.disease Trophoblasts Up-Regulation MicroRNAs 030104 developmental biology medicine.anatomical_structure Apoptosis 030220 oncology & carcinogenesis Cancer research biology.protein Female RNA Long Noncoding |
| DOI: | 10.6084/m9.figshare.14453036 |
| Popis: | Background: Preeclampsia (PE) is an idiopathic hypertensive disorder of pregnancy, which is related to abnormal placental villi development. Our previous study has found that lncRNA NEAT1 promotes apoptosis of trophoblasts, but the role of NEAT1 in proliferation, migration, and invasion is still unclear. This study explores the role of NEAT1 in proliferation, migration, and invasion of trophoblasts. Methods: NEAT1 and miR-411-5p levels were detected by quantitative real-time PCR. Colony formation assay detected cell proliferation and transwell assay detected cell migration and invasion. Dual-luciferase reporter assay detected the binding between NEAT1 and miR-411-5p as well as the binding between miR-411-5p and PTEN. RNA pull-down assay detected the combination between NEAT1 and miR-411-5p. Result: NEAT1 was increased and miR-411-5p was reduced in PE patients and human trophoblasts (HTR8/SVneo cells) that were induced with H2O2. Interference with NEAT1 promoted cell proliferation, migration, and invasion, and the miR-411-5p inhibitor reversed the effect of siRNA-NEAT1. The expression of PTEN was promoted in PE patients and HTR8/SVneo cells that were induced with H2O2, while the miR-411-5p mimic inhibited PTEN expression, and the plasmid-mediated PTEN overexpression reversed the effect of the miR-411-5p mimic. Besides, under H2O2 induction, the miR-411-5p mimic promoted cell proliferation, migration, and invasion, and the plasmid-mediated PTEN overexpression reversed the effect of the miR-411-5p mimic. Conclusion: Interference with lncRNA NEAT1 promoted the proliferation, migration, and invasion of trophoblasts and alleviated the development of PE, which was partly mediated by upregulating miR-411-5p and inhibiting PTEN expression. |
| Databáze: | OpenAIRE |
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