Regulation of the ferredoxin component of renal hydroxylases at transcriptional and postranslational levels and of the protein inhibitor of cyclic AMP-dependent kinase
| Autor: | Raymond K. Blanchard, Gail S. Marchetto, Helen L. Henry, Chilung Tang |
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| Rok vydání: | 1995 |
| Předmět: |
Transcription
Genetic Endocrinology Diabetes and Metabolism medicine.medical_treatment Clinical Biochemistry Biology Kidney Biochemistry Gene Expression Regulation Enzymologic Endocrinology Protein biosynthesis medicine Animals Phosphorylation Protein kinase A Molecular Biology Protein Kinase C Ferredoxin 25-Hydroxyvitamin D3 1-alpha-Hydroxylase Adrenal ferredoxin Kinase Intracellular Signaling Peptides and Proteins Cell Biology Cyclic AMP-Dependent Protein Kinases Mitochondria Steroid hormone medicine.anatomical_structure Protein Biosynthesis Ferredoxins Tetradecanoylphorbol Acetate Molecular Medicine Signal transduction Carrier Proteins Chickens Signal Transduction |
| Zdroj: | The Journal of Steroid Biochemistry and Molecular Biology. 53:595-598 |
| ISSN: | 0960-0760 |
| DOI: | 10.1016/0960-0760(95)00105-9 |
| Popis: | We have studied two proteins potentially involved in the regulation of the 25-OH-D-1-hydroxylase, which is located in the renal mitochondria and which is responsible for the production of the steroid hormone 1,25(OH)2D3. The endogenous inhibitor of cyclic AMP-dependent protein kinase, PKI, is down regulated by 1,25(OH)2D3. Having cloned and sequenced PKI cDNA, we studied its message levels and found them to be regulated by 1,25(OH)2D3 tissue specifically in the kidney and in kidney cell culture. In other experiments we over expressed the ferredoxin component of the 1-hydroxylase and found it to be physically and chemically indistinguishable from those of classic steroidogenic tissues. The mRNA encoding the ferredoxin component is up-regulated by chronic vitamin D deficiency, which at the same time leads to sustained elevation in 1-hydroxylase activity; no short term effect of 1,25(OH)2D3 on ferredoxin mRNA in kidney cell culture could be demonstrated. Finally, there was an association between decreased phosphorylation of ferredoxin and decreased 1-hydroxylase activity brought about by treatment of cultured kidney cells with TPA. Control of the renal signaling events involved in the production of 1,25(OH)2D3 remains a fruitful area of investigation in the field of the metabolism and actions of vitamin D and its metabolites. |
| Databáze: | OpenAIRE |
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