Decreasing the hyphal branching rate ofSaccharopolyspora erythraea NRRL 2338 leads to increased resistance to breakage and increased antibiotic production
| Autor: | John N. Wardell, Colin R. Thomas, Michael E. Bushell, Stuart M. Stocks |
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| Rok vydání: | 2002 |
| Předmět: |
Pseudonocardiaceae
Hypha Mutant Hyphae Bioengineering Biology Branching (polymer chemistry) Sensitivity and Specificity Applied Microbiology and Biotechnology Microbiology Bioreactors Breakage Drug Resistance Bacterial Image Processing Computer-Assisted fungi Reproducibility of Results biology.organism_classification Erythromycin Fermentation Mutation Saccharopolyspora erythraea Stress Mechanical Bacteria Saccharopolyspora Biotechnology |
| Zdroj: | Biotechnology and Bioengineering. 78:141-146 |
| ISSN: | 1097-0290 0006-3592 |
| DOI: | 10.1002/bit.10210 |
| Popis: | Mutation and selection for increased resistance to cell-wall synthesis inhibitors led to alterations in the hyphal branching rate of Saccharopolyspora erythraea NRRL 2338. Mutants with decreased branching frequency exhibited increased hyphal strength (estimated by in vitro micromanipulation). As the hyphal strength was increased, this led to a greater proportion of hyphal particles in liquid culture with a hyphal fragment diameter of greater than 88 microm. This, in turn, coincided with proportionately increased antibiotic production. |
| Databáze: | OpenAIRE |
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