COUP-TFII Stimulates Dentin Sialophosphoprotein Expression and Mineralization in Odontoblasts
| Autor: | Sun-Ouck Kim, Yun-Chan Hwang, Je-Hwang Ryu, Jin Woong Kim, Byung-Chul Jeong, Kkot-Nim Lee, Sung-Woong Hur, Hyuck Choi, Sin-Hye Oh, J.T. Koh |
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| Rok vydání: | 2015 |
| Předmět: |
Small interfering RNA
medicine.medical_specialty Sialoglycoproteins Blotting Western Chicken ovalbumin upstream promoter-transcription factor Real-Time Polymerase Chain Reaction Transfection COUP Transcription Factor II Rats Sprague-Dawley Mice stomatognathic system Dentin sialophosphoprotein Internal medicine medicine Animals Humans Immunoprecipitation General Dentistry Transcription factor Cells Cultured COUP-TFII Homeodomain Proteins Extracellular Matrix Proteins Gene knockdown Odontoblasts Staining and Labeling Chemistry Cell Differentiation Dentinogenesis Phosphoproteins Rats Cell biology stomatognathic diseases Endocrinology Odontoblast Nuclear receptor |
| Zdroj: | Journal of Dental Research. 94:1135-1142 |
| ISSN: | 1544-0591 0022-0345 |
| DOI: | 10.1177/0022034515585125 |
| Popis: | Chicken ovalbumin upstream promoter transcription factor 2 (COUP-TFII), an orphan nuclear receptor belonging to the steroid-thyroid hormone receptor superfamily, plays an important role in cell fate determination of various tissues. However, the specific role of COUP-TFII in tooth development has not yet been elucidated. In the present study, we aimed to explore the role of COUP-TFII in dentin sialophosphoprotein ( DSPP) expression and matrix mineralization in odontoblast-lineage cells. In primary human dental pulp cells (HDPCs) and murine dental papilla-derived cells (MDPC-23) cultured in a mineralizing medium, the expression of COUP-TFII was induced along with the increased odontoblast-specific dentin matrix protein-1 ( DMP-1) and DSPP expression. Endogenous expression of COUP-TFII in maxillary second molar germs of rats showed an increasing tendency as development of the tooth progressed. Also, COUP-TFII protein was detected in greater quantity in the odontoblastic layer of second molar germs than in that of third molar germs of rats. Overexpression of COUP-TFII using an adenoviral system upregulated the expression of odontoblast-specific genes with increased alkaline phosphatase activity and matrix mineralization in odontoblast-lineage cells. In contrast, knockdown of COUP-TFII using small interfering RNA decreased the expression of odontoblast-specific genes, which reduced matrix mineralization. Mechanistic studies revealed that COUP-TFII increased DSPP transcription by direct binding on the DSPP promoter. In addition, COUP-TFII physically interacted with the homeodomain transcription factor Msx2 and antagonistically regulated the Msx2 effect on DSPP promoter activity. Taken together, these results suggest that COUP-TFII has a stimulatory role in DSPP expression and matrix mineralization in odontoblast-lineage cells. |
| Databáze: | OpenAIRE |
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