Catalase activity as a predictor of amniotic fluid culture results in preterm labor or premature rupture of membranes
| Autor: | William M. Janda, Guillermo Font, Thomas D. Myles, Andre Bieniarz, William J. Meyer, Daniel W. Gauthier |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
medicine.medical_specialty
Fetal Membranes Premature Rupture Amniotic fluid Gestational Age Prom Gastroenterology Sensitivity and Specificity law.invention Obstetric Labor Premature law Predictive Value of Tests Pregnancy Internal medicine Fetal distress Medicine Humans medicine.diagnostic_test Staining and Labeling business.industry Obstetrics and Gynecology Gestational age medicine.disease Amniotic Fluid Catalase Gram staining Endocrinology Amniocentesis Female Anaerobic bacteria business Premature rupture of membranes Ureaplasma urealyticum |
| Zdroj: | Obstetrics and gynecology. 85(5 Pt 1) |
| ISSN: | 0029-7844 |
| Popis: | Objective: To evaluate catalase activity as a rapid predictor of microbial invasion of amniotic fluid (AF). Methods: The study population consisted of 74 patients before 36 weeks' gestation with preterm labor or premature rupture of membranes (PROM). Subjects were excluded if there was evidence of clinical chorioamnionitis or fetal distress at admission. Amniocentesis was done within 24 hours of admission, and the AF was cultured for aerobic and anaerobic bacteria and for Mycoplasma species. All AF samples were Gram stained, and slides were examined by microbiology technologists. Amniotic fluid catalase activity was measured immediately after amniocentesis using a commercially available kit. The sensitivity of the Gram stain and catalase activity were compared using McNemar exact test. Results: Amniotic fluid cultures were positive in 12 of 37 (32%) patients presenting with preterm labor and in 21 of 37 (56%) patients with PROM. Catalase activity was significantly more sensitive than Gram stain in detecting positive AF cultures in cases of PROM (P Conclusion: Catalase activity is a simple, rapid test that is useful in identifying subclinical intra-amniotic infection in patients with preterm labor or PROM. |
| Databáze: | OpenAIRE |
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