KAP1 facilitates reinstatement of heterochromatin after DNA replication
| Autor: | Geneviève Almouzni, Andrea Coluccio, Didier Trono, Jean-Pierre Quivy, Julien Pontis, Priscilla Turelli, Benjamin Rauwel, Annamaria Kauzlaric, Julien Duc, Suk Min Jang, Sandra Offner |
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| Přispěvatelé: | Ecole Polytechnique Fédérale de Lausanne (EPFL), Université Paris sciences et lettres (PSL), Dynamique du noyau [Institut Curie], Institut Curie [Paris]-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Sorbonne Université - Faculté de Médecine (SU FM), Sorbonne Université (SU) |
| Rok vydání: | 2018 |
| Předmět: |
DNA Replication
0301 basic medicine DNA repair Heterochromatin Tripartite Motif-Containing Protein 28 Mice 03 medical and health sciences Proliferating Cell Nuclear Antigen [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN] Genetics Animals Humans Epigenetics Phosphorylation Cells Cultured biology MCM6 Gene regulation Chromatin and Epigenetics DNA replication Methyltransferases Chromatin Assembly and Disassembly Chromatin Proliferating cell nuclear antigen Cell biology Repressor Proteins HEK293 Cells 030104 developmental biology Histone NIH 3T3 Cells biology.protein K562 Cells Protein Processing Post-Translational HeLa Cells |
| Zdroj: | Nucleic Acids Research Nucleic Acids Research, Oxford University Press, 2018, 46 (17), pp.8788-8802. ⟨10.1093/nar/gky580⟩ |
| ISSN: | 1362-4962 0305-1048 |
| Popis: | International audience; During cell division, maintenance of chromatin features from the parental genome requires their proper establishment on its newly synthetized copy. The loss of epigenetic marks within heterochromatin, typically enriched in repetitive elements, endangers genome stability and permits chromosomal rearrangements via recombination. However, how histone modifications associated with heterochromatin are maintained across mitosis remains poorly understood. KAP1 is known to act as a scaffold for a repressor complex that mediates local heterochromatin formation, and was previously demonstrated to play an important role during DNA repair. Accordingly, we investigated a putative role for this protein in the replication of heterochromatic regions. We first found that KAP1 associates with several DNA replication factors including PCNA, MCM3 and MCM6. We then observed that these interactions are promoted by KAP1 phosphorylation on serine 473 during S phase. Finally, we could demonstrate that KAP1 forms a complex with PCNA and the histone-lysine methyltransferase Suv39h1 to reinstate heterochromatin after DNA replication. |
| Databáze: | OpenAIRE |
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