The adaptor proteins HAP1a and GRIP1 collaborate to activate kinesin-1 isoform KIF5C
| Autor: | Erika L.F. Holzbaur, Alison E. Twelvetrees, Flavie Lesept, Josef T. Kittler |
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| Rok vydání: | 2019 |
| Předmět: |
Microtubule transport
Gene isoform Molecular motor Protein subunit Short Report Kinesins Nerve Tissue Proteins macromolecular substances Biology Autoinhibition Microtubules Motor protein 03 medical and health sciences 0302 clinical medicine Adaptor proteins Chlorocebus aethiops Animals Humans Protein Isoforms 030304 developmental biology 0303 health sciences Signal transducing adaptor protein Kinesin Cell Biology In vitro Cell biology COS Cells Hinge region Carrier Proteins 030217 neurology & neurosurgery HeLa Cells Protein Binding |
| Zdroj: | Journal of Cell Science |
| ISSN: | 1477-9137 0021-9533 |
| DOI: | 10.1242/jcs.215822 |
| Popis: | Binding of motor proteins to cellular cargoes is regulated by adaptor proteins. HAP1 and GRIP1 are kinesin-1 adaptors that have been implicated individually in the transport of vesicular cargoes in the dendrites of neurons. We find that HAP1a and GRIP1 form a protein complex in the brain, and co-operate to activate the kinesin-1 subunit KIF5C in vitro. Based upon this co-operative activation of kinesin-1, we propose a modification to the kinesin activation model that incorporates stabilisation of the central hinge region known to be critical to autoinhibition of kinesin-1. Summary: The adaptor proteins HAP1a and GRIP1 form a protein complex in the brain, and co-operate to activate the kinesin-1 subunit KIF5C in vitro. |
| Databáze: | OpenAIRE |
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