Plastidic protein Cdf1 is essential in Arabidopsis embryogenesis
| Autor: | Masayuki Kakimoto, Minoru Nagano, Maki Kawai-Yamada, Hirofumi Uchimiya |
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| Rok vydání: | 2013 |
| Předmět: |
Genetics
Heterozygote biology Cell growth Arabidopsis Proteins Embryogenesis Mutant Genetic Complementation Test Arabidopsis Embryo Plant Science biology.organism_classification Plants Genetically Modified Green fluorescent protein Cell biology Repressor Proteins Guard cell Mutation Seeds Plastids Plastid Carrier Proteins Promoter Regions Genetic |
| Zdroj: | Planta. 239(1) |
| ISSN: | 1432-2048 |
| Popis: | Arabidopsis cell growth defect factor-1 (Cdf1 in yeast, At5g23040) was originally isolated as a cell growth suppressor of yeast from genetic screening. To investigate the in vivo role of Cdf1 in plants, a T-DNA insertion line was analyzed. A homozygous T-DNA insertion mutant (cdf1/cdf1) was embryo lethal and showed arrested embryogenesis at the globular stage. The Cdf1 protein, when fused with green fluorescent protein, was localized to the plastid in stomatal guard cells and mesophyll cells. A promoter-β-glucuronidase assay found expression of Cdf1 in the early heart stage of embryogenesis, suggesting that Cdf1 was essential for Arabidopsis embryogenesis during the transition of the embryo from the globular to heart stage. |
| Databáze: | OpenAIRE |
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