An Enzyme-Linked Immunosorbent Assay(ELISA) for the Detection of Antibody against Streptococcus suis Type 2 in Infected Pigs
| Autor: | Muneo Nakazawa, Toshiharu Yamashita, Seiji Sunaga, Yasushi Kataoka, Hitoshi Ishikawa, Yumiko Imada, Masato Kishima |
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| Rok vydání: | 1996 |
| Předmět: |
Serotype
Streptococcus suis Swine animal diseases Enzyme-Linked Immunosorbent Assay medicine.disease_cause Sensitivity and Specificity Microbiology Antigen Predictive Value of Tests Reference Values Streptococcal Infections medicine Animals Swine Diseases chemistry.chemical_classification General Veterinary biology Polysaccharides Bacterial Cross reactions Pathogenic bacteria biology.organism_classification Antibodies Bacterial Enzyme chemistry Elisa test biology.protein Antibody |
| Zdroj: | Journal of Veterinary Medical Science. 58:369-372 |
| ISSN: | 1347-7439 0916-7250 |
| DOI: | 10.1292/jvms.58.369 |
| Popis: | An ELISA test for the detection of antibody against S. suis type 2 in pigs was developed and applied to field sera. The best sensitivity and specificity of ELISA were obtained when a purified polysaccharide antigen was used. It showed no cross reaction with sera against other serotypes of S. suis and other pathogenic bacteria. A total of 264 sera were collected from 20 pig farms and examined with the antibody against S. suis type 2. In the affected farms, 17.0% of pigs tested were positive, 9.8% in the adjacent farms, but only 3.4% in the free farms. The difference of the positive percentages between the affected and the free farms was statistically significant (P0.05). |
| Databáze: | OpenAIRE |
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