An optimised neurobehavioural observation battery integrated with the assessment of cardiovascular function in the beagle dog
| Autor: | Marcello Tontodonati, Alessandra Giarola, Nicola Fasdelli, Elena Moscardo, Roberto Dorigatti |
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| Rok vydání: | 2009 |
| Předmět: |
Male
Neurobehavioural Observation Battery Cardiovascular response Radio-telemetry medicine.medical_specialty Blood Pressure Neurological examination Motor Activity Reflex Pupillary Toxicology Beagle Body Temperature Cardiovascular Physiological Phenomena Electrocardiography Dogs Physical medicine and rehabilitation Heart Rate Heart rate medicine Animals Telemetry Neurologic Examination Pharmacology Video recording Behavior Animal medicine.diagnostic_test business.industry Safety pharmacology Remote observation Video image Blood pressure Anesthesia Models Animal business |
| Zdroj: | Journal of Pharmacological and Toxicological Methods. 60:198-209 |
| ISSN: | 1056-8719 |
| DOI: | 10.1016/j.vascn.2009.05.005 |
| Popis: | Introduction The objective of this study was to describe an optimised neurobehavioural observation battery in the beagle dog and to demonstrate that it can be used for an integrated assessment of cardiovascular and neurobehavioural functions within a single Safety Pharmacology study. Methods A standardised and detailed observation battery was established based on the direct examination of 44 signs, including behavioural responses and a full, systematic neurological examination, using clearly defined numerical scores. To complete the neurobehavioural assessment the remote observation of animals by video recording was also performed. Results from two studies, where cardiovascular and neurobehavioural parameters were assessed using drugs that either showed or did not show cardiovascular effects, are presented to show the influence of the direct neurobehavioural exam on the telemetrically acquired cardiovascular parameters. Results Heart rate and systolic blood pressure were affected by the handling required for the neurobehavioural procedure for a total of approximately 25 min, consisting in 20 min needed for the sequential neurobehavioural observation of four dogs (requiring approximately 5 min each) and approximately 5 min to return to baseline. Drug related cardiovascular effects were not affected by the changes associated with these neurobehavioural observations. More evident cardiovascular changes were observed with other routine procedures such as feeding. Discussion The direct neurobehavioural examination caused fluctuations of the telemetric cardiovascular parameters for no more than 5 min from the end of the procedure and this did not alter or jeopardise the analysis and interpretation of the cardiovascular parameters. These results confirmed that this optimised neurobehavioural observation battery can be used in the beagle dog for a reliable integrated assessment of neurobehavioural and cardiovascular changes during the course of Safety Pharmacology evaluations. |
| Databáze: | OpenAIRE |
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