Detection of Carp Interstitial Nephritis and Gill Necrosis Virus in Fish Droppings
| Autor: | Shlomit Werker, Moshe Kotler, Maya Ilouze, Ayana Perelberg, Maya Davidovich, Janette Bishara-Shieban, Arnon Dishon |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Carps
viruses Cyprinid herpesvirus 3 ved/biology.organism_classification_rank.species Antibodies Viral medicine.disease_cause Polymerase Chain Reaction Applied Microbiology and Biotechnology Herpesviridae Virus law.invention Feces Fish Diseases Common carp law Environmental Microbiology medicine Animals Carp Cells Cultured Polymerase chain reaction Ecology biology ved/biology DNA Viruses Brain DNA virus biology.organism_classification Virology DNA Virus Infections Alloherpesviridae DNA Viral Nephritis Interstitial Food Science Biotechnology |
| Zdroj: | Applied and Environmental Microbiology. 71:7285-7291 |
| ISSN: | 1098-5336 0099-2240 |
| Popis: | Carp interstitial nephritis and gill necrosis virus (CNGV) is an unclassified large DNA virus that morphologically resembles members of the Herpesviridae but contains a large (ca. ∼280-kbp) linear double-stranded DNA. This virus has also been named koi herpesvirus, koi herpes-like virus, and cyprinid herpesvirus 3. CNGV is the cause of a lethal disease that afflicts common carp and koi. By using immunohistochemistry, molecular analysis, and electron microscopy we previously demonstrated that this virus is present mainly in the intestine and kidney of infected fish. Based on these observations, we postulated that viruses and/or viral components may appear in droppings of infected carp. Here we report that (i) by using PCR we demonstrated that fish droppings contain viral DNA, (ii) fish droppings contain viral antigens which are useful for CNGV diagnosis, and (iii) fish droppings contain active virus which can infect cultured common carp brain cells and induce the disease in naïve fish following inoculation. Thus, our findings show that CNGV can be identified by using droppings without taking biopsies or killing fish and that infectious CNGV is present in the stools of sick fish. The possibility that fish droppings preserve viable CNGV during the nonpermissive seasons is discussed. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|