Evaluation of Matrix Effects in Quantifying Microbial Secondary Metabolites in Indoor Dust Using Ultraperformance Liquid Chromatograph–Tandem Mass Spectrometer
| Autor: | Mukhtar Jaderson, Ju-Hyeong Park |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Dust sample
Analyte Chemical Health and Safety Chromatography Tandem Chemistry lcsh:Public aspects of medicine Extraction (chemistry) Public Health Environmental and Occupational Health lcsh:RA1-1270 Mass spectrometry 030210 environmental & occupational health Matrix (chemical analysis) 03 medical and health sciences 0302 clinical medicine Original Article 030212 general & internal medicine Safety Risk Reliability and Quality Safety Research |
| Zdroj: | Safety and Health at Work, Vol 10, Iss 2, Pp 196-204 (2019) |
| ISSN: | 2093-7911 |
| Popis: | Background: Liquid chromatography–tandem mass spectrometry (LC-MSMS) for simultaneous analysis of multiple microbial secondary metabolites (MSMs) is potentially subject to interference by matrix components. Methods: We examined potential matrix effects (MEs) in analyses of 31 MSMs using ultraperformance LC-MSMS. Twenty-one dust aliquots from three buildings (seven aliquots/building) were spiked with seven concentrations of each of the MSMs (6.2 pg/μl–900 pg/μl) and then extracted. Another set of 21 aliquots were first extracted and then, the extract was spiked with the same concentrations. We added deepoxy-deoxynivalenol (DOM) to all aliquots as a universal internal standard. Ten microliters of the extract was injected into the ultraperformance LC-MSMS. ME was calculated by subtracting the percentage of the response of analyte in spiked extract to that in neat standard from 100. Spiked extract results were used to create a matrix-matched calibration (MMC) curve for estimating MSM concentration in dust spiked before extraction. Results: Analysis of variance was used to examine effects of compound (MSM), building and concentration on response. MEs (range: 63.4%–99.97%) significantly differed by MSM (p |
| Databáze: | OpenAIRE |
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