Modulation of differentiation in vitro
| Autor: | C. Bournoutian, A. Macieira-Coelho, J. P. Wahrmann, D. Delain |
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| Rok vydání: | 1981 |
| Předmět: |
Population
Plant Science Myosins Biology Cell Line Cell Fusion Mice Myosin Animals Myocyte Polylysine education education.field_of_study Myogenesis Muscles Cell Cycle Cytarabine DNA replication Cell Differentiation DNA In vitro Cell biology Polyglutamic Acid Cell culture Developmental biology Biotechnology |
| Zdroj: | In Vitro. 17:752-762 |
| ISSN: | 1475-2689 0073-5655 |
| DOI: | 10.1007/bf02618441 |
| Popis: | Myogenic cells of the L6 line proliferate and fuse in culture to form myotubes that actively synthesize muscle-specific proteins such as myosin. We show that the expression of the differentiated phenotype can be influenced by the electrical charges of the substratum on which the cells were grown. Negatively charged surfaces did not influence the developmental program of the cells although positively charged ones interfered with myogenesis. The interaction operates primarily by interfering with the mitotic cycle, which is slowed down, with fusion which is blocked, and with myosin synthesis, which is reduced. Our results show that growth of the cells on positively charged surfaces prevents the switching of a large fraction of the population from a proliferative state to a differentiating program. We postulate that this interference might operate through the slowdown in DNA replication. The cell culture method described represents a good model for studying the different steps involved in the differentiation of L6 cells. |
| Databáze: | OpenAIRE |
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