Pathogenesis of Acute Viral Disease Induced in Fish by Carp Interstitial Nephritis and Gill Necrosis Virus
| Autor: | Marina Hutoran, Michael Steinitz, Ayana Perelberg, Moshe Kotler, Yechiam Shapira, Ariel Ronen, Berta Levavi-Sivan, Julia Abramowitz, Dov Soffer, Eli Pikarsky |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Gills
Gill Carps Fish farming Interstitial nephritis Cyprinid herpesvirus 3 Immunology ved/biology.organism_classification_rank.species Biology Kidney Microbiology Virus Fish Diseases Common carp Cytopathogenic Effect Viral Virology medicine Animals Cloning Molecular Carp Cells Cultured ved/biology medicine.disease biology.organism_classification Immunohistochemistry Kinetics Microscopy Electron Virus Diseases Insect Science Acute Disease DNA Viral Viruses Nephritis Interstitial Pathogenesis and Immunity Genetic Engineering Nephritis |
| Zdroj: | Journal of Virology. 78:9544-9551 |
| ISSN: | 1098-5514 0022-538X |
| Popis: | A lethal disease of koi and common carp (species Cyprinus carpio ) has afflicted many fish farms worldwide since 1998, causing severe financial losses. Morbidity and mortality are restricted to common carp and koi and appear in spring and autumn, when water temperatures are 18 to 28°C. We have isolated the virus causing the disease from sick fish, propagated it in koi fin cell culture, and shown that virus from a single clone causes lethal disease in carp and koi upon infection. Intraperitoneal virus injection or bathing the fish in virus-containing water kills 85 to 100% of the fish within 7 to 21 days. This virus is similar to the previously reported koi herpesvirus; however, it has characteristics inconsistent with the herpesvirus family, and thus we have called it carp interstitial nephritis and gill necrosis virus. We examined the pathobiology of this disease in carp by using immunohistochemistry and PCR. We found large amounts of the virus in the kidneys of sick fish and smaller amounts in liver and brain. A rapid increase in the viral load in the kidneys was detected by using both immunofluorescence and semiquantitative PCR. Histological analyses of fish at various times after infection revealed signs of interstitial nephritis as early as 2 days postinfection, which increased in severity up to 10 days postinfection. There was severe gill disease evidenced by loss of villi with accompanying inflammation in the gill rakers. Minimal focal inflammation was noted in livers and brains. This report describes the etiology and pathology of a recently described viral agent in fish. |
| Databáze: | OpenAIRE |
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