Enterococci populations of a metropolitan river after an extreme flood event: prevalence, persistence and virulence determinants
| Autor: | Jasmin M. Thompson, Eva Hatje, Tara L. Vollmerhausen, N Masters, Aaron Wiegand, Mohammad Katouli |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Veterinary medicine medicine.drug_class 030106 microbiology Antibiotics Virulence Drug resistance Biology Microbiology Persistence (computer science) 03 medical and health sciences Antibiotic resistance Rivers medicine Typing Waste Management and Disposal Water Science and Technology High prevalence Flood myth Public Health Environmental and Occupational Health Drug Resistance Microbial biochemical phenomena metabolism and nutrition Floods Infectious Diseases Phenotype Biofilms Queensland Enterococcus |
| Zdroj: | Journal of water and health. 15(5) |
| ISSN: | 1477-8920 |
| Popis: | We investigated the prevalence, persistence and virulence determinants of enterococci populations in water samples collected over three rounds following an extreme flood event in a metropolitan river. Enterococci (n = 482) were typed using the high resolution biochemical fingerprinting method (PhP typing) and grouped into common (C) or single (S) biochemical phenotypes (BPTs). In all, 23 C-BPTs (72.6% of isolates) were found across the sites. A representative isolate of each C-BPT was identified to the species level and tested for the presence of seven virulence genes (VGs), biofilm formation and resistance to 14 antibiotics. The enterococci concentrations in samples collected during the first two rounds were above national recreational water guidelines. By round three, enterococci concentrations decreased significantly (P < 0.05). However, 11 C-BPTs (55.5% of isolates) persisted across all sampling rounds. E. casseliflavus and E. mundtii were the most common enterococci populations comprising of >57% of all isolates. Ten of the 11 most dominant C-BPTs were resistant to multiple antibiotics and harboured one or more VGs. The high prevalence of antibiotic resistance and VGs among enterococci isolates in this catchment not only provides them with niche advantages but also poses a risk to public health. |
| Databáze: | OpenAIRE |
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