Herpes simplex virus 1 induces de novo phospholipid synthesis
| Autor: | Andres Kaech, Mathias Ackermann, Elisabeth M. Schraner, Sabrina Sonda, Miriam S. Lucas, Kurt Tobler, Esther Sutter, Anna Paula de Oliveira, Peter J. Wild |
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| Přispěvatelé: | University of Zurich, Wild, Peter |
| Rok vydání: | 2012 |
| Předmět: |
Nuclear membranes
10078 Institute of Parasitology Nuclear Envelope 10077 Institute of Veterinary Anatomy Phospholipid 610 Medicine & health Herpesvirus 1 Human Vacuole Biology Tritium medicine.disease_cause Choline chemistry.chemical_compound symbols.namesake 600 Technology Virology Chlorocebus aethiops medicine Animals Herpes virus Golgi membranes Vero Cells Phospholipids Cellular compartment 10217 Clinic for Visceral and Transplantation Surgery Morphometry Endoplasmic reticulum Cell Membrane Golgi apparatus Phospholipid synthesis Cell biology Microscopy Electron Membrane Herpes simplex virus chemistry Intracellular traffic Cytoplasm Isotope Labeling Host-Pathogen Interactions SEM TEM symbols 2406 Virology 570 Life sciences biology 10244 Institute of Virology |
| Zdroj: | Virology. 429(2):124-135 |
| ISSN: | 0042-6822 |
| DOI: | 10.1016/j.virol.2012.04.004 |
| Popis: | Herpes simplex virus type 1 capsids bud at nuclear membranes and Golgi membranes acquiring an envelope composed of phospholipids. Hence, we measured incorporation of phospholipid precursors into these membranes, and quantified changes in size of cellular compartments by morphometric analysis. Incorporation of [3H]-choline into both nuclear and cytoplasmic membranes was significantly enhanced upon infection. [3H]-choline was also part of isolated virions even grown in the presence of brefeldin A. Nuclei expanded early in infection. The Golgi complex and vacuoles increased substantially whereas the endoplasmic reticulum enlarged only temporarily. The data suggest that HSV-1 stimulates phospholipid synthesis, and that de novo synthesized phospholipids are inserted into nuclear and cytoplasmic membranes to i) maintain membrane integrity in the course of nuclear and cellular expansion, ii) to supply membrane constituents for envelopment of capsids by budding at nuclear membranes and Golgi membranes, and iii) to provide membranes for formation of transport vacuoles. |
| Databáze: | OpenAIRE |
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