Monitoring the Effects of Chiral Pharmaceuticals on Aquatic Microorganisms by Metabolic Fingerprinting
| Autor: | Roger M. Jarvis, Royston Goodacre, Emma S. Wharfe, Catherine L. Winder |
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| Rok vydání: | 2010 |
| Předmět: |
Antimetabolites
Physiology Microorganism medicine.disease_cause Applied Microbiology and Biotechnology High-performance liquid chromatography Pseudomonas Spectroscopy Fourier Transform Infrared medicine Chromatography High Pressure Liquid Ecology biology Pseudomonas aeruginosa Metabolism biology.organism_classification Propranolol Pseudomonas putida Sphingomonadaceae Micrococcus luteus Atenolol Biochemistry Metabolome Enantiomer Bacteria Food Science Biotechnology |
| Zdroj: | Applied and Environmental Microbiology. 76:2075-2085 |
| ISSN: | 1098-5336 0099-2240 |
| DOI: | 10.1128/aem.02395-09 |
| Popis: | The effects of the chiral pharmaceuticals atenolol and propranolol on Pseudomonas putida , Pseudomonas aeruginosa , Micrococcus luteus , and Blastomonas natatoria were investigated. The growth dynamics of exposed cultures were monitored using a Bioscreen instrument. In addition, Fourier-transform infrared (FT-IR) spectroscopy with appropriate chemometrics and high-performance liquid chromatography (HPLC) were employed in order to investigate the phenotypic changes and possible degradation of the drugs in exposed cultures. For the majority of the bacteria studied there was not a statistically significant difference in the organism's phenotype when it was exposed to the different enantiomers or mixtures of enantiomers. In contrast, the pseudomonads appeared to respond differently to propranolol, and the two enantiomers had different effects on the cellular phenotype. This implies that there were different metabolic responses in the organisms when they were exposed to the different enantiomers. We suggest that our findings may indicate that there are widespread effects on aquatic communities in which active pharmaceutical ingredients are present. |
| Databáze: | OpenAIRE |
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