LncRNA DLG2-AS1 as a Novel Biomarker in Lung Adenocarcinoma
| Autor: | Paola Peinado, Daniel J García, María Isabel Rodríguez, Alberto M Arenas, Juan Carlos Álvarez-Pérez, Marta Cuadros, Pedro P. Medina, Carlos Baliñas-Gavira, Alvaro Andrades, Isabel F. Coira |
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| Přispěvatelé: | [Arenas,AM, Andrades,A, Coira,IF, Baliñas-Gavira,C, Peinado,P, Álvarez-Pérez,JC, Medina,PP] Department of Biochemistry and Molecular Biology I, Faculty of Sciences, University of Granada, Granada, Spain. [Arenas,AM, Cuadros,M, García,DJ, Rodríguez,MI, Medina,PP] GENYO, Centre for Genomics and Oncological Research, Pfizer/University of Granada/Andalusian Regional Government, Granada, Spain. [Cuadros,M, Rodríguez,MI] Department of Biochemistry and Molecular Biology III and Immunology, Faculty of Medicine, University of Granada, Granada, Spain. [Cuadros,M, Medina,PP] Health Research Institute of Granada (ibs.Granada), Granada, Spain., Medina’s lab was funded by the Spanish Ministry of Economy and Business under grant SAF2015-67919-R, the Consejería de Salud de la Junta de Andalucía under grant Pl-0245-2017, CS2016-3, the Asociación Española Contra el Cáncer (AECC) Foundation under grant LabAECC2018, and the International Association for the Study of Lung Cancer (IASLC)’s Young Investigator Award 2017. A.M.A. and A.A. were supported by a Spanish Ministry of Education, Culture and Sports FPU fellowship (FPU17/01258, and FPU17/00067, respectively). D.G.J. was supported by a 'Fundación Benéfica Anticáncer Santa Cándida y San Francisco Javier' predoctoral fellowship. I.F.C. was supported by a Spanish Ministry of Economy and Business FPI fellowship (BES-2013-064596). P.P. was supported by a 'La Caixa Foundation' fellowship (LCF/BQ/DE15/10360019). J.C.A.P. was supported by a Marie Sklodowska Curie Actions postdoctoral fellowship (H2020-MSCA-IF-2018, 837897). |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
ARN largo no codificante
0301 basic medicine Cancer Research tumor Chemicals and Drugs::Nucleic Acids Nucleotides and Nucleosides::Nucleic Acids::RNA::RNA Untranslated::RNA Long Noncoding [Medical Subject Headings] Biology Diseases::Neoplasms::Neoplasms by Site::Thoracic Neoplasms::Respiratory Tract Neoplasms::Lung Neoplasms [Medical Subject Headings] Phenomena and Processes::Genetic Phenomena::Genetic Processes::Gene Expression [Medical Subject Headings] lcsh:RC254-282 Article Long noncoding adenocarcinoma of lung Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans [Medical Subject Headings] 03 medical and health sciences 0302 clinical medicine Anatomy::Cells::Cells Cultured::Cell Line [Medical Subject Headings] Transcription (biology) Gene expression Adenocarcinoma del pulmón medicine Epigenetics Lung cancer Phenomena and Processes::Biological Phenomena [Medical Subject Headings] Tumor Analytical Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Statistics as Topic::Area Under Curve [Medical Subject Headings] Phenomena and Processes::Genetic Phenomena::Genetic Processes::Gene Expression Regulation::Epigenesis Genetic [Medical Subject Headings] RNA biomarkers Anatomy::Respiratory System::Lung [Medical Subject Headings] medicine.disease lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens Neoplasias 3. Good health ARN long noncoding Biomarcadores 030104 developmental biology Oncology 030220 oncology & carcinogenesis Adenocarcinoma of lung Cancer research Adenocarcinoma Biomarker (medicine) Chemicals and Drugs::Biological Factors::Biological Markers::Biomarkers Pharmacological [Medical Subject Headings] Biomarkers Overlapping gene |
| Zdroj: | Cancers, Vol 12, Iss 2080, p 2080 (2020) Digibug. Repositorio Institucional de la Universidad de Granada instname Digibug: Repositorio Institucional de la Universidad de Granada Universidad de Granada (UGR) Cancers Volume 12 Issue 8 |
| ISSN: | 2072-6694 |
| Popis: | Long non-coding RNAs (lncRNAs) are a heterogeneous class of non-coding RNAs whose biological roles are still poorly understood. LncRNAs serve as gene expression regulators, frequently interacting with epigenetic factors to shape the outcomes of crucial biological processes, and playing roles in di erent pathologies including cancer. Over the last years, growing scientific evidence supports the key role of some lncRNAs in tumor development and proposes them as valuable biomarkers for the clinic. In this study, we aimed to characterize lncRNAs whose expression is altered in tumor samples from patients with lung adenocarcinoma (LUAD) compared to adjacent normal tissue samples. On an RT-qPCR survey of 90 cancer-related lncRNAs, we found one lncRNA, DLG2-AS1, which was consistently downregulated in 70 LUAD patients. To gain insight into its biological function, DLG2-AS1 was cloned and successfully re-expressed in LUAD cancer cell lines. We determined that DLG2-AS1 is not a cis-regulatory element of its overlapping gene DLG2, as their transcription levels were not correlated, nor did DLG2-AS1 restoration modify the expression of DLG2 protein. Furthermore, after generating a receiver operating curve (ROC) and calculating the area under curve (AUC), we found that DLG2-AS1 expression showed high sensitivity and specificity (AUC = 0.726) for the classification of LUAD and normal samples, determining its value as a potential lung cancer biomarker. Spanish Ministry of Economy and Business SAF2015-67919-R Junta de Andalucía CS2016-3 Pl-0245-2017 Asociación Española Contra el Cáncer (AECC) Foundation LabAECC2018 International Association for the Study of Lung Cancer (IASLC)'s Young Investigator Award 2017 Spanish Ministry of Education, Culture and Sports FPU fellowship FPU17/01258 FPU17/00067 "Fundacion Benefica Anticancer Santa Candida y San Francisco Javier" predoctoral fellowship Spanish Ministry of Economy and Business FPI fellowship BES-2013-064596 La Caixa Foundation LCF/BQ/DE15/10360019 Marie Sklodowska Curie Actions postdoctoral fellowship (H2020-MSCA-IF-2018) 837897 |
| Databáze: | OpenAIRE |
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