Oligosaccharides from Polygonatum Cyrtonema Hua: Structural characterization and treatment of LPS-induced peritonitis in mice
| Autor: | Caiyun Yao, Zhifeng Zhang, Lili He, Bing-Xiong Yan, Zhijun Song, Long-Wei Li, Pei Luo, Xiao-Yi Chen, Song Shanshan, Youjiao Wu |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Lipopolysaccharides
Male Polymers and Plastics Lipopolysaccharide Acute Lung Injury Interleukin-1beta Anti-Inflammatory Agents Gene Expression Oligosaccharides Peritonitis 02 engineering and technology Lung injury 010402 general chemistry 01 natural sciences Monocytes Mice chemistry.chemical_compound Fructan Cell Movement In vivo Materials Chemistry medicine Animals Humans Polygonatum cyrtonema Lung Mice Inbred BALB C Tumor Necrosis Factor-alpha Chemistry Atomic force microscopy Fructooligosaccharide Polygonatum Organic Chemistry 021001 nanoscience & nanotechnology medicine.disease Survival Analysis Molecular biology 0104 chemical sciences Disease Models Animal 0210 nano-technology Drugs Chinese Herbal |
| Zdroj: | Carbohydrate Polymers. 255:117392 |
| ISSN: | 0144-8617 |
| DOI: | 10.1016/j.carbpol.2020.117392 |
| Popis: | Fructooligosaccharide was isolated from Polygonatum Cyrtonema Hua (PFOS) for the first time. Structure characterized using FT-IR, MALDI-TOF-MS, NMR, AFM, and TEM, indicated that PFOS was graminan-type fructan with a degree of polymerization ranging from 5 to 10. A murine model of lipopolysaccharide (LPS)-induced peritonitis was used to evaluate the in vivo anti-inflammatory and lung protective efficacy of PFOS. The result shown that pretreatment with PFOS (1.0 mg/mL) in peritonitis-induced mice could significantly inhibit the level of pro-inflammatory cytokines (TNF-α, IL-1β) in serum (P 0.001), increase mice survival rate from 12.5 % to 54 % (P0.05), and alleviated lung injury through ameliorating the damage of the pulmonary cellular architecture and reducing inflammatory monocyte accumulation in lung tissue. This effect of oligosaccharides could explain the traditional usage of P. cyrtonema as a tonic medicine for respiratory problems and it could be used as a potential natural ingredient with anti-inflammatory activity. |
| Databáze: | OpenAIRE |
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