Fe limitation decreases transcriptional regulation over the diel cycle in the model diatom Thalassiosira pseudonana

Autor: Mónica V. Orellana, Chris T. Berthiaume, Sacha Coesel, E. Virginia Armbrust, Johanna A. L. Goldman, Megan J. Schatz
Jazyk: angličtina
Rok vydání: 2019
Předmět:
0106 biological sciences
0301 basic medicine
Metabolic Processes
Atmospheric Science
Light
Gene Expression
01 natural sciences
Biochemistry
Gene expression
Transcriptional regulation
Regulation of gene expression
Multidisciplinary
biology
Chemistry
Physics
Electromagnetic Radiation
Eukaryota
Plants
Plankton
Cell biology
Physical Sciences
Medicine
Glycolysis
Research Article
Algae
Science
Iron
Photoperiod
Thalassiosira pseudonana
DNA transcription
Phosphates
03 medical and health sciences
Greenhouse Gases
Genetics
Animals
Environmental Chemistry
Gene Regulation
14. Life underwater
Diel vertical migration
Gene
Diatoms
fungi
Ecology and Environmental Sciences
Organisms
Chemical Compounds
Biology and Life Sciences
Metabolism
Carbon Dioxide
biology.organism_classification
Invertebrates
030104 developmental biology
Diatom
Gene Expression Regulation
Atmospheric Chemistry
Phytoplankton
Earth Sciences
Transcriptome
010606 plant biology & botany
Zdroj: PLoS ONE
PLoS ONE, Vol 14, Iss 9, p e0222325 (2019)
ISSN: 1932-6203
Popis: Iron (Fe) is an important growth factor for diatoms and its availability is further restricted by changes in the carbonate chemistry of seawater. We investigated the physiological attributes and transcriptional profiles of the diatom Thalassiosira pseudonana grown on a day: night cycle under different CO2/pH and iron concentrations, that in combination generated available iron (Fe') concentrations of 1160, 233, 58 and 12 pM. We found the light-dark conditions to be the main driver of transcriptional patterns, followed by Fe' concentration and CO2 availability, respectively. At the highest Fe' (1160 pM), 55% of the transcribed genes were differentially expressed between day and night, whereas at the lowest Fe' (12 pM), only 28% of the transcribed genes displayed comparable patterns. While Fe limitation disrupts the diel expression patterns for genes in most central metabolism pathways, the diel expression of light- signaling molecules and glycolytic genes was relatively robust in response to reduced Fe'. Moreover, we identified a non-canonical splicing of transcripts encoding triose-phosphate isomerase, a key-enzyme of glycolysis, generating transcript isoforms that would encode proteins with and without an active site. Transcripts that encoded an active enzyme maintained a diel expression at low Fe', while transcripts that encoded the non-active enzyme lost the diel expression. This work illustrates the interplay between nutrient limitation and transcriptional regulation over the diel cycle. Considering that future ocean conditions will reduce the availability of Fe in many parts of the oceans, our work identifies some of the regulatory mechanisms that may shape future ecological communities.
Databáze: OpenAIRE
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