Comparison of Extraction Methods and Extraction Optimization in Aqueous Two-Phase System for Ethyl Carbamate Analysis in Model Systems for Maesil Wine
| Autor: | Tabu Mungia Magollah, Hyo-Rim Kim, Ji-Yun Ko, Su-Yeon Park, Seo-Yeon Kwon, Yu-Kyeong Kim, Ji-Hyo Lee, Yong-Soo Seo, Ji-Young Yang, Yang-Bong Lee |
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| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | Preventive Nutrition and Food Science. 27:234-240 |
| ISSN: | 2287-8602 2287-1098 |
| Popis: | Ethyl carbamate (EC) is a known carcinogen, and therefore its intake is regulated internationally. The objectives of this study were to compare the EC recovery yields under different liquid-liquid extraction (LLE) conditions and to investigate the optimum conditions of the aqueous two-phase system (ATPS) for EC extraction. Our results showed that for the LLE method, addition of 15% NaCl improved the EC yield by 15%, and dichloromethane as the extraction solvent showed a slightly higher yield (about 5%) than chloroform. However, there was little difference in the yield when mixing was performed using an ultrasonic bath compared to a vortex mixer. Using response surface methodology with central composite design to analyze the ATPS results, optimal extraction was found to occur at 21.5°C for 2.8 h in the sample containing 70% alcohol and 15% phosphate, showing a recovery yield of 75.64%. This information can be applied to alcoholic beverages and other fermented food products to analyze EC with better extraction methods, depending on the types of food. |
| Databáze: | OpenAIRE |
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