Physical Training Is a Potential Modifier of Risk for Contrast-Induced Acute Kidney Injury in Diabetes Mellitus

Autor: Adriana Almeida de Souza, Karina B Peres, Sheila Marques Fernandes Couto, Vinicius Cardoso da Silva, Beatriz de Almeida Brandi, Carolina Conde, Douglas Ikedo Machado, Maria de Fátima Fernandes Vattimo
Rok vydání: 2020
Předmět:
Male
medicine.medical_specialty
Article Subject
DIABETES MELLITUS
Urinary system
030232 urology & nephrology
Urology
Contrast Media
Renal function
Hemodynamics
030204 cardiovascular system & hematology
Kidney
Kidney Function Tests
urologic and male genital diseases
Thiobarbituric Acid Reactive Substances
General Biochemistry
Genetics and Molecular Biology
Diabetes Mellitus
Experimental
Diabetic nephropathy
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Risk Factors
Physical Conditioning
Animal
Diabetes mellitus
Animals
Medicine
Sulfhydryl Compounds
Rats
Wistar
Creatinine
Nitrates
General Immunology and Microbiology
business.industry
Acute kidney injury
General Medicine
Acute Kidney Injury
medicine.disease
Peroxides
chemistry
Renal blood flow
business
Oxidation-Reduction
Research Article
Zdroj: Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP
BioMed Research International
BioMed Research International, Vol 2020 (2020)
ISSN: 2314-6141
2314-6133
DOI: 10.1155/2020/1830934
Popis: Background. Iodinated contrast (IC) is a leading cause of hospital-based acute kidney injury (AKI). Contrast-induced acute kidney injury (CI-AKI) is a decline in renal function due to iodinated contrast administration and occurs more frequently in individuals with increasingly common risk factors, such as diabetes mellitus (DM). Physical training (PT) can have renoprotective effects on CI-AKI in diabetic nephropathy. The aim of this study was to evaluate the injury in kidneys of diabetic rats submitted to treatment with IC, evaluating the impact of PT on hemodynamics and renal function in addition to oxidative profile in diabetic rats submitted to IC-AKI. Materials and Methods. Adult male Wistar rats are randomized into four groups: citrate ( n = 7 ): control group, citrate buffer (streptozotocin-STZ vehicle), intravenous tail (iv), single dose; DM ( n = 7 ): STZ, 60 mg/kg, iv, single dose; DM+IC ( n = 7 ): DM rats treated with IC (sodium meglumine ioxithalamate, 6 mL/kg, intraperitoneal (ip), single dose); DM+IC+PT ( n = 7 ): DM rats treated with IC as mentioned and submitted to physical training. Renal function parameters (inulin clearance, neutrophil gelatinase-associated lipocalin (NGAL), serum creatinine, and urinary albumin), hemodynamics (renal blood flow and renal vascular resistance), and oxidative profile (urinary peroxides, urinary TBARS, urinary nitric oxide, and renal tissue thiols) were evaluated. Results. It was possible to observe a decrease in inulin clearance, renal blood flow, and thiols in renal tissue accompanied by an increase in urinary flow, serum creatinine, urinary albumin, renal vascular resistance, urinary peroxides, urinary nitrate, and TBARS in the DM group compared to the citrate group. The DM+IC group showed a reduction in inulin clearance, and the renal dysfunction was also seen by the increased NGAL. Renal hemodynamics and oxidative profile compared were also worsened in the DM group. PT improved renal function by increasing renal blood flow and thiol levels in renal tissue and reduced renal vascular resistance, metabolites of reactive oxygen, nitrogen species, and lipid peroxidation in the DM+IC+PT group compared to DM+IC. Conclusions. Our results confirmed that DM induction increases renal vulnerability to the toxicity of IC and an association between DM with IC predisposes to severe AKI with reduced renal function alongside with renal hemodynamic alterations and oxidative mechanism of injury. The PT showed a renoprotective effect in DM animals subjected to damage with IC by modulating renal hemodynamics and oxidative profile, confirming a potential to modify the risk of CI-AKI when diabetes mellitus is present.
Databáze: OpenAIRE
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