Exhaustive Multi-Parametric Assessment of the Behavioral Array of Daily Activities of Mice Using Cluster and Factor Analysis
| Autor: | Camille Simard, Kenzo Yamamoto, Daphné Houle-Martel, Denis Gris, Theodore Cloutier, Katsiaryna V. Gris, Shaimaa Mahmoud, Marjan Gharagozloo, Jesús E. Sotelo Fonseca, Pavel Gris |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Activities of daily living social isolation Cognitive Neuroscience Machine learning computer.software_genre supervised learning behavioral assessment lcsh:RC321-571 03 medical and health sciences Behavioral Neuroscience 0302 clinical medicine medicine Social isolation lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry R program Original Research Multi parametric business.industry homecagescan Supervised learning Behavioral assessment Behavioral pattern automated behavioral assessment long-term continuous recording Hierarchical clustering acclimatization 030104 developmental biology Neuropsychology and Physiological Psychology Home cage Artificial intelligence medicine.symptom Psychology business computer 030217 neurology & neurosurgery Neuroscience |
| Zdroj: | Frontiers in Behavioral Neuroscience, Vol 12 (2018) Frontiers in Behavioral Neuroscience |
| ISSN: | 1662-5153 |
| DOI: | 10.3389/fnbeh.2018.00187/full |
| Popis: | Using automated supervised behavioral assessment software, we recorded and analyzed 24 h non-interrupted recordings of mice for a duration of 11 days. With the assistance of free R programming, we used correlation matrix-based hierarchical clustering and factor analysis to separate the 33 activities into meaningful clusters and groups without losing the exhaustive nature of the findings. These groups represent novel meaningful behavioral patterns exhibited by mice in home cage. Thirty-three activities were separated into 5 clusters based on dissimilarity between activities and 6 factors based on statistical modeling. Using these two methods, we describe and compare behavioral arrays of two groups of animals: 1. Continuously recorded for 11 days in social isolation and 2. Intermittently socially isolated for recording on days 1, 3, 5, 8, and 10, while socializing on the other days. This is the first work to our knowledge that interprets mouse home cage activities throughout a 24 h period and proposes a base line of a daily routine of a healthy C57Bl/6J mouse that can be used for various experimental paradigms, including disease, neuroinflammation, or drug testing to trace behavioral changes that follow intervention. In this work, we defined the necessary acclimatization period for the 24 h recording paradigm of home cage behavior. We demonstrated the behavioral changes that are associated with the effect of social isolation, intermittent socialization, and re-introduction to a familiar home cage. We provide the full description of the codes used in R. |
| Databáze: | OpenAIRE |
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