Sampling forest soils to describe fungal diversity and composition. Which is the optimal sampling size in Mediterranean pure and mixed pine oak forests?
| Autor: | Juan Martínez de Aragón, Javier Parladé, Irene Adamo, José Antonio Bonet, Carles Castaño, Yasmine Piñuela, Josu G. Alday, Joan Pera |
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| Přispěvatelé: | Producció Vegetal, Protecció Vegetal Sostenible |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
Mediterranean climate Soil test Beta diversity Sample (statistics) Forests Biology 01 natural sciences DNA metabarcoding Quercus 03 medical and health sciences Forest ecology Genetics Mediterranean forest DNA Fungal Sample pooling Soil Microbiology Ecology Evolution Behavior and Systematics 030304 developmental biology 2. Zero hunger 0303 health sciences Mixed forests Fungi Sampling (statistics) Beta-diversity Forestry Biodiversity 15. Life on land Pinus Fungal communities Infectious Diseases Soil water Species richness Environmental Monitoring 010606 plant biology & botany |
| Zdroj: | Fungal Biology Repositorio Abierto de la UdL Universitad de Lleida Recercat. Dipósit de la Recerca de Catalunya instname IRTA Pubpro. Open Digital Archive Institut de Recerca i Tecnologia Agroalimentàries (IRTA) Recercat: Dipósit de la Recerca de Catalunya Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| Popis: | Soil sampling is a critical step affecting perceived fungal diversity, however sampling optimization for high-throughput-DNA sequencing studies have never been tested in Mediterranean forest ecosystems. We identified the minimum number of pooled samples needed to obtain a reliable description of fungal communities in terms of diversity and composition in three different Mediterranean forests (pine, oak, and mixed-pine-oak). Twenty soil samples were randomly selected in each of the three plots per type. Samples were pooled to obtain mixtures of 3, 6, 10, 15, 20 samples, and sequenced using Illumina MiSeq of fungal ITS2 amplicons. Pooling three soil samples in Pinus and Quercus stands provided consistent richness estimations, while at least six samples were needed in mixed-stands. ß-diversity decreased with increasing sample pools in monospecific-stands, while there was no effect of sample pool size on mixed35 stands. Soil sample pooling had no effect over species composition. We estimate that three samples would be already optimal to describe fungal richness and composition in Mediterranean pure stands, while at least six samples would be needed in mixed stands. This work was partially supported by the Spanish Ministry of Science, Innovation and Universities, grants AGL2015-66001-C3, RTI2018-093907-B-C21 and RTI2018-099315-A-I00. I.A. was supported by a Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie Cofund grant agreement No. 801596, J.G.A. was supported by Ramon y Cajal fellowship (RYC-2016-20528) and J.A.B. benefitted from a Serra-Húnter Fellowship provided by the Generalitat of Catalunya. |
| Databáze: | OpenAIRE |
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