Conditioned Medium from Activated Rat Macrophages and the Recombinant Factors, IL-1β and GM-CSF, Enhance the Accessory Activity of Dendritic Cells
| Autor: | Mary S. Ruhoff, William E. Bowers, Estelle M. Goodell |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Male
Time Factors medicine.medical_treatment Immunology Biology Tritium Biological Factors Colony-Stimulating Factors medicine Animals Immunology and Allergy Macrophage Growth Substances Antigen-presenting cell Granulocyte-Macrophage Colony-Stimulating Factor Dendritic Cells Hematology Dendritic cell T lymphocyte Macrophage Activation Silicon Dioxide Molecular biology Recombinant Proteins Culture Media Rats Granulocyte macrophage colony-stimulating factor Cytokine Rats Inbred Lew Cell culture Chromatography Gel Female Tumor necrosis factor alpha Interleukin-1 Thymidine medicine.drug |
| Zdroj: | Immunobiology. 180:362-384 |
| ISSN: | 0171-2985 |
| DOI: | 10.1016/s0171-2985(11)80299-8 |
| Popis: | Low density lymph node cells (LD-LNC; 5% of total unfractionated LNC) contain 95% of the accessory activity required for responses of T lymphocytes to mitogens. Significantly greater responses to mitogens occur when T lymphocytes are added to LD-LNC that have been exposed overnight to silica, in comparison to responses occurring with LD-LNC incubated without silica. Conditioned medium (CM) from silica-treated LD-LNC is itself able to mediate enhanced responses; i.e., when LD-LNC are exposed overnight to CM alone and mitogen-treated T lymphocytes added the next day. The enhancing activity found in CM from LD-LNC exposed to silica is produced by macrophages; however, their low accessory activity is not enhanced by CM. In contrast, dendritic cells isolated from LD-LNC exposed to silica or to CM show significantly increased accessory activity, but dendritic cells do not produce the enhancing activity found in CM. CM lacks IL-2 activity and does not have any effect on the responses of untreated or mitogen-treated T lymphocytes alone. Thus, macrophages produce the enhancing activity and dendritic cells respond to it. Maximum enhancement of dendritic cell accessory activity requires overnight exposure to CM; once induced, accessory activity is not further modulated after continued incubation in the presence or absence of CM. LD-LNC, adherent peritoneal exudate cells, and adherent thioglycollate-induced peritoneal exudate cells produce enhancing activity after exposure to silica, LPS, and silica plus LPS. After gel filtration of a CM produced by silica plus LPS, enhancing activity shows a broad molecular weight distribution between 20 and 55 kD. IL-1 is present in CM and shows a more narrow molecular weight distribution that falls within the lower molecular weight range for enhancing activity. Silica treatment by itself produces CM containing little IL-1, but abundant enhancing activity; gel filtration of this CM shows that the distribution of enhancing activity is confined more narrowly to the higher molecular weight range, suggesting that IL-1 is one of several factors that enhances the accessory activity of dendritic cells. Recombinant human IL-1 beta does have enhancing activity, but of the other recombinant factors tested only mouse GM-CSF also has enhancing activity. Human IL-1 alpha, tumor necrosis factor alpha, IL-4, rat IL-3 and rat IFN-gamma, as well as L cell-conditioned medium containing M-CSF, lack enhancing activity. |
| Databáze: | OpenAIRE |
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