FURTHER OBSERVATIONS ON THE URINARY EXCRETION OF WATER-SOLUBLE CORTICOSTEROIDS BY NORMAL AND ABNORMAL SUBJECTS*
| Autor: | M. S. Wood, A. M. Campbell, E. Christo, Nathan B. Talbot, J. Worcester, Aniela S. Zygmuntowicz |
|---|---|
| Rok vydání: | 1951 |
| Předmět: |
medicine.medical_specialty
Preservative Chloroform Chromatography Chemistry Endocrinology Diabetes and Metabolism Biochemistry (medical) Clinical Biochemistry Extraction (chemistry) Water Urine Biochemistry chemistry.chemical_compound Endocrinology Water soluble Urinary excretion Adrenal Cortex Hormones Reagent Healthy individuals Internal medicine Adrenal Cortex medicine Humans Glucocorticoids |
| Zdroj: | The Journal of Clinical Endocrinology & Metabolism. 11:1224-1236 |
| ISSN: | 1945-7197 0021-972X |
| DOI: | 10.1210/jcem-11-11-1224 |
| Popis: | INTRODUCTION IN PREVIOUS papers, 300 preliminary observations on the urinary excretion of water-soluble corticosteroids by sick and healthy individuals have been reported (1, 2). The present communication records an additional approximately 350 measurements and summarizes studies carried out to date. The data presented indicate that this measurement serves as a reasonably reliable index of relative rates of adrenocortical 11-17-oxycorticosteroid production. METHODS Urines were collected without preservative and stored in the refrigerator. They were extracted and assayed according to a procedure described previously (3), which involves chloroform extraction of fresh urine adjusted to pH 6.5 and extensive purification of the extracts by alkali washing, partitioning between benzene and water and ketonic separation with Girard's reagent. The final measurement is based upon a copper-reduction type of assay, as used for the determination of sugar. Because this procedure appears to eliminate from the urine reduc... |
| Databáze: | OpenAIRE |
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