Rapid translocation of pluripotency-related transcription factors by external uniaxial forces
Autor: | Paul H. Krebsbach, Cheri X. Deng, Byoung Choul Kim, Tuğba Topal, Shuichi Takayama, Luis G. Villa-Diaz |
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Rok vydání: | 2019 |
Předmět: |
Homeobox protein NANOG
Mechanotransduction General Science & Technology Integrin Biophysics Stem Cell Research - Embryonic - Non-Human Bioengineering Oct4 Regenerative Medicine CRM1 Nanog Biochemistry Medicinal and Biomolecular Chemistry 03 medical and health sciences 0302 clinical medicine SOX2 stem cells Transcription factor 030304 developmental biology 0303 health sciences Matrigel FAK biology Chemistry Pharmacology and Pharmaceutical Sciences Stem Cell Research Embryonic stem cell Cell biology Stem cell fate determination hESCs biology.protein cytoplasmic translocation Original Article Generic health relevance Biochemistry and Cell Biology Stem cell 030217 neurology & neurosurgery |
Zdroj: | Integrative biology : quantitative biosciences from nano to macro, vol 11, iss 2 |
ISSN: | 1757-9708 |
Popis: | Human embryonic stem cells subjected to a one-time uniaxial stretch for as short as 30-min on a flexible substrate coated with Matrigel experienced rapid and irreversible nuclear-to-cytoplasmic translocation of NANOG and OCT4, but not Sox2. Translocations were directed by intracellular transmission of biophysical signals from cell surface integrins to nuclear CRM1 and were independent of exogenous soluble factors. On E-CADHERIN-coated substrates, presumably with minimal integrin engagement, mechanical strain-induced rapid nuclear-to-cytoplasmic translocation of the three transcription factors. These findings might provide fundamental insights into early developmental processes and may facilitate mechanotransduction-mediated bioengineering approaches to influencing stem cell fate determination. |
Databáze: | OpenAIRE |
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