Flavonoid Fraction of Citrus Reticulata Juice Reduces Proliferation and Migration of Anaplastic Thyroid Carcinoma Cells

Autor:	Roberta Francesca De Rose, Diego Russo, Michele Navarra, Valentina Maggisano, Stefania Bulotta, Jessica Maiuolo, Marilena Celano
Rok vydání:	2015
Předmět:	Citrus Cancer Research medicine.medical_specialty Cell cycle checkpoint Cell Flavonoid Medicine (miscellaneous) Antineoplastic Agents Pharmacology Biology Thyroid Carcinoma Anaplastic Cell Line Thyroid carcinoma Cell Movement Cell Line Tumor Internal medicine Autophagy medicine Anaplastic Humans Thyroid Neoplasms Thyroid cancer Cell Cycle Checkpoints Cell Death Cell Proliferation Flavonoids Fruit and Vegetable Juices Matrix Metalloproteinase 2 Plant Extracts Nutrition and Dietetics Oncology Medicine (all) chemistry.chemical_classification Tumor Cell growth Thyroid Carcinoma Cell cycle medicine.disease Antineoplastic Agents Phytogenic medicine.anatomical_structure Endocrinology chemistry Cell culture
Zdroj:	Nutrition and Cancer. 67:1183-1190
ISSN:	1532-7914 0163-5581
Popis:	Effects of flavonoids extracted from Citrus reticulata (mandarin) juice on proliferation and migration of 3 human anaplastic thyroid carcinoma (ATC) cell lines were evaluated. Flavonoid components of Mandarin juice extract (MJe) were analyzed by uHPLC. Proliferation of CAL-62, C-643, and 8505C cells, measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, was significantly reduced by MJe in a concentration- and time-dependent way, with maximal effect elicited at 0.5 mg/ml concentration after 48 h. Cytofluorimetric analysis showed a block in the G2/M phase of the cell cycle, accompanied by low cell mortality owed to autophagic death. The extract caused also a reduction of cell migration, associated with decreased activity of the metalloproteinase MMP-2. These findings demonstrate that the flavonoid fraction of mandarin juice exerts in vitro antiproliferative effects on ATC cells, associated with a reduction of migration, suggesting for such a functional food a potential use as adjuvant in the treatment of thyroid cancer.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9d149c2cbd59bbadf0620c78e44eadbc https://doi.org/10.1080/01635581.2015.1073760 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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