Analysis of risk factors and prevalence of haemoplasma infection in dogs
| Autor: | Chelsea A E Hicks, Séverine Tasker, Giane Regina Paludo, J. Kamani, Larissa C. Aquino, Christopher R Helps, A. M. Haruna |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Male 040301 veterinary sciences 030106 microbiology Nigeria medicine.disease_cause 0403 veterinary science 03 medical and health sciences Dogs Mycoplasma Risk Factors RNA Ribosomal 16S Sequence Homology Nucleic Acid Genetic variation medicine Prevalence Animals Mycoplasma Infections Genetic variability Dog Diseases Phylogeny General Veterinary biology Anaplasma ovis Genetic Variation 04 agricultural and veterinary sciences General Medicine biology.organism_classification 16S ribosomal RNA Virology Anaplasma phagocytophilum Haemoplasmas Mycoplasma haemofelis Risk factors Aerococcus Parasitology Female |
| Zdroj: | Aquino, L C, Kamani, J, Haruna, A M, Paludo, G R, Hicks, C A, Helps, C R & Tasker, S 2016, ' Analysis of risk factors and prevalence of haemoplasma infection in dogs ', Veterinary Parasitology, vol. 221, pp. 111-117 . https://doi.org/10.1016/j.vetpar.2016.03.014 |
| ISSN: | 1873-2550 |
| Popis: | Mycoplasma haemocanis (Mhc) and 'Candidatus Mycoplasma haematoparvum' (CMhp) are canine haemoplasma species that can induce anaemia in immunocompromised and/or splenectomised dogs. This study aimed to determine the prevalence and phylogeny of canine haemoplasma species in dogs from Nigeria and describe any risk factors for infection. Canine haemoplasma species-specific and generic haemoplasma qPCR assays were used. The species-specific qPCR assays found Mhc infection in 18 of 245 dogs (7.3%), and CMhp infection in only one dog (0.4%). The generic haemoplasma qPCR assays were positive in 44 of 245 (17.9%) dogs. Twenty-five dogs had discordant qPCR results in that they were generic haemoplasma qPCR positive but species-specific qPCR negative. Further evaluation of these dogs by 16S rDNA sequencing gave limited results but 5 were confirmed to be infected with non-haemoplasma species: 2 Anaplasma phagocytophilum, 1 Anaplasma ovis, 1 Serratia marcescens and 1 Aerococcus spp. The 16S rRNA gene sequences from Mhc species showed > 99.8% identity with each other and > 99.6% identity with GenBank sequences, and resided in a single clade with other global Mhc and Mycoplasma haemofelis sequences, indicating low 16S rRNA genetic variability amongst this canine haemoplasma species. |
| Databáze: | OpenAIRE |
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