Integration of MsrB1 and MsrB2 in the Redox Network during the Development of Orthodox and Recalcitrant Acer Seeds
| Autor: | Agnieszka Bagniewska-Zadworna, Pascal Rey, Karolina Bilska, Ewa Marzena Kalemba, Natalia Wojciechowska, Ewelina Stolarska |
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| Přispěvatelé: | Polish Academy of Sciences (PAN), Adam Mickiewicz University in Poznań (UAM), Protéines de Protection des Végétaux (PPV), Institut de Biosciences et Biotechnologies d'Aix-Marseille (ex-IBEB) (BIAM), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), National Science Center (Poland) Grant No. 2015/18/E/NZ9/00729, Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Physiology Clinical Biochemistry seed maturation Acer platanoides Nicotinamide adenine dinucleotide Protein oxidation 01 natural sciences Biochemistry Redox Article 03 medical and health sciences chemistry.chemical_compound methionine sulfoxide Botany Acer pseudoplatanus [SDV.BV]Life Sciences [q-bio]/Vegetal Biology glutathione Molecular Biology biology Chemistry Methionine sulfoxide lcsh:RM1-950 Cell Biology 15. Life on land ascorbate biology.organism_classification nicotinamide adenine dinucleotide phosphate lcsh:Therapeutics. Pharmacology 030104 developmental biology methionine sulfoxide reductase Methionine sulfoxide reductase NAD+ kinase 010606 plant biology & botany |
| Zdroj: | Antioxidants Antioxidants, 2020, 9 (12), pp.1250. ⟨10.3390/antiox9121250⟩ Antioxidants, MDPI, 2020, 9 (12), pp.1250. ⟨10.3390/antiox9121250⟩ Volume 9 Issue 12 Antioxidants, Vol 9, Iss 1250, p 1250 (2020) |
| ISSN: | 2076-3921 |
| DOI: | 10.3390/antiox9121250⟩ |
| Popis: | International audience; Two related tree species, Norway maple (Acer platanoides L.) and sycamore (Acer pseudoplatanus L.), produce desiccation-tolerant (orthodox) and desiccation-sensitive (recalcitrant) seeds, respectively. We compared the seeds of these two species to characterize the developmentally driven changes in the levels of peptide-bound methionine sulfoxide (MetO) and the abundance of methionine sulfoxide reductases (Msrs) B1 and B2, with respect to the cellular redox environment. Protein oxidation at the Met level was dynamic only in Norway maple seeds, and the reduced MsrB2 form was detected only in this species. Cell redox status, characterized by the levels of reduced and oxidized ascorbate, glutathione, and nicotinamide adenine dinucleotide (NAD)/phosphate (NADP), was clearly more reduced in the Norway maple seeds than in the sycamore seeds. Clear correlations between MetO levels, changes in water content and redox status were reported in orthodox Acer seeds. The abundance of Msrs was correlated in both species with redox determinants, mainly ascorbate and glutathione. Our data suggest that MsrB2 is associated with the acquisition of desiccation tolerance and that ascorbate might be involved in the redox pathway enabling the regeneration of Msr via intermediates that are not known yet. |
| Databáze: | OpenAIRE |
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