A novel small RNA S042 increases acid tolerance in Lactococcus lactis F44
| Autor: | Binbin Wang, Shunyi Song, Kairen Tian, Jianjun Qiao, Hao Wu, Hongji Zhu, Jiaheng Liu, Zhou Dandan |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Small RNA Arginine Green Fluorescent Proteins 030106 microbiology Biophysics Biochemistry 03 medical and health sciences Genes Reporter Transcription (biology) Transferase RNA Messenger Northern blot Molecular Biology Gene Base Sequence biology Sequence Analysis RNA Chemistry Lactococcus lactis Reproducibility of Results Gene Expression Regulation Bacterial Cell Biology Hydrogen-Ion Concentration biology.organism_classification Adaptation Physiological RNA Bacterial 030104 developmental biology Genes Bacterial Transfer RNA Acids |
| Zdroj: | Biochemical and Biophysical Research Communications. 500:544-549 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2018.04.069 |
| Popis: | Lactococcus lactis, a gram-positive bacterium, encounters various environmental stresses, especially acid stress, during fermentation. Small RNAs (sRNAs) that serve as regulators at post-transcriptional level play important roles in acid stress response. Here, a novel sRNA S042 was identified by RNA-Seq, RT-PCR and Northern blot. The transcription level of s042 was upregulated 2.29-fold under acid stress by Quantitative RT-PCR (qRT-PCR) analysis. Acid tolerance assay showed that overexpressing s042 increased the survival rate of L. lactis F44 and deleting s042 significantly inhibited the viability under acidic conditions. Moreover, the targets were predicted by online software and four genes were chosen as candidates. Among them, argR (arginine regulator) and accD (acetyl-CoA carboxylase carboxyl transferase subunit beta) were validated to be the direct targets activated by S042 through reporter fusion assay. The regulatory mechanism between S042 and its targets was further investigated through Bioinformatics and qRT-PCR. This study served to highlight the role of the novel sRNA S042 in acid resistance of L. lactis and provided new insights into the response mechanism of acid stress. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect