Monitoring intracellular redox potential changes using SERS nanosensors
| Autor: | Patricia Richardson, Colin Campbell, Hamish McNab, Craig Auchinvole, Catherine McGuinnes, Ken Donaldson, Venkatesh Mallikarjun |
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| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Cytoplasm
General Physics and Astronomy Nanotechnology Biosensing Techniques Spectrum Analysis Raman medicine.disease_cause Redox symbols.namesake Nanosensor medicine General Materials Science Particle Size Redox homeostasis Chemistry SERS General Engineering Equipment Design intracellular Nanoshell Nanostructures Equipment Failure Analysis Intracellular potential redox symbols Gold nanosensor Raman spectroscopy Oxidation-Reduction Intracellular Oxidative stress |
| Zdroj: | Auchinvole, C, Richardson, P, McGuinnes, C, Mallikarjun, V, Donaldson, K, McNab, H & Campbell, C 2012, ' Monitoring intracellular redox potential changes using SERS nanosensors ', ACS Nano, vol. 6, no. 1, pp. 888-896 . https://doi.org/10.1021/nn204397q |
| DOI: | 10.1021/nn204397q |
| Popis: | Redox homeostasis and signaling are critically important in the regulation of cell function. There are significant challenges in quantitatively measuring intracellular redox potentials, and in this paper, we introduce a new approach. Our approach is based on the use of nanosensors which comprise molecules that sense the local redox potential, assembled on a gold nanoshell. Since the Raman spectrum of the sensor molecule changes depending on its oxidation state and since the nanoshell allows a huge enhancement of the Raman spectrum, intracellular potential can be calculated by a simple optical measurement. The nanosensors can be controllably delivered to the cytoplasm, without any toxic effects, allowing redox potential to be monitored in a reversible, non-invasive manner over a previously unattainable potential range encompassing both superphysiological and physiological oxidative stress. |
| Databáze: | OpenAIRE |
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