Effects of growth factors and hormones on basal and FSH-stimulated inhibin production by porcine granulosa cells in vitro
| Autor: | Uwe Michel, W Wuttke, S Ludemann, H Jarry |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
endocrine system
medicine.medical_specialty Swine medicine.medical_treatment Reproductive technology Biology Oxytocin Dinoprostone Gonadotropin-Releasing Hormone 03 medical and health sciences Follicle-stimulating hormone 0302 clinical medicine Endocrinology Transforming Growth Factor beta Internal medicine Genetics medicine Animals Inhibins Insulin-Like Growth Factor I Growth Substances Molecular Biology Cells Cultured 030304 developmental biology Platelet-Derived Growth Factor 0303 health sciences Granulosa Cells 030219 obstetrics & reproductive medicine Epidermal Growth Factor Estradiol Inhibin secretion Growth factor Androstenedione Hormones Prolactin Arginine Vasopressin Reproductive Medicine Female Animal Science and Zoology Folliculogenesis Follicle Stimulating Hormone Spermatogenesis hormones hormone substitutes and hormone antagonists Developmental Biology Biotechnology Hormone |
| Zdroj: | Reproduction, Fertility and Development. 3:201 |
| ISSN: | 1031-3613 |
| DOI: | 10.1071/rd9910201 |
| Popis: | The effect of several growth factors, protein and steroid hormones on follicle stimulating hormone (FSH)-stimulated and basal inhibin secretion by mature porcine granulosa cells (g-cells) in culture was examined in order to elucidate the putative role of growth factors and hormones in the regulation of inhibin secretion by porcine g-cells in vitro. Cells were incubated with the respective hormones over a timespan of 0-144 h and immunoreactive inhibin was measured with a radioimmunoassay against porcine inhibin. Epidermal growth factor (EGF) and human transforming growth factor type beta (TGF-beta) decreased basal and gonadotrophin-stimulated inhibin and progesterone in a dose-dependent manner. In the absence of insulin, insulin-like growth factor type I (IGF-I) caused a 4-fold enhancement of basal inhibin secretion, but inhibin secretion was elevated only to 20% above control in the presence of 500 nM insulin. Porcine platelet-derived growth factor (PDGF) had no significant effect on basal or FSH-induced inhibin secretion by g-cells. In addition, neither gonadotrophin-releasing hormone (GnRH) nor prolactin (PRL), arginine vasopressin (AVP) and oxytocin affected basal or FSH-stimulated inhibin release by porcine g-cells. Oestradiol caused a slight but significant (P less than 0.01) rise of basal inhibin production (158% of control) in the last 2 days of culture (96-144 h) and the effect of androstenedione on basal (158% of control) and FSH-stimulated (140% of control) inhibin release (P less than 0.01) was also only visible on Days 4-6 of culture. In contrast to androstenedione and oestradiol, progesterone did not show any effect during 6 days of culture in a dose range of 10(-5) to 10(-9) M. Like steroids, prostaglandin E2 (PGE2) had a stimulatory effect on basal inhibin production (250% of control) by porcine g-cells, visible on Days 3-6 of culture, but an inhibitory effect on FSH-stimulated release (less than 40% of control). Over all the experiments with different hormones and growth factors, tested in varying doses and over a time span of 0-144 h, there was a strong correlation between progesterone and inhibin secretion by g-cells (0-48 h = 0.78; 48-96 h = 0.92; 96-144 h = 0.92). These results suggest that EGF, TGF-beta, IGF-I, oestradiol and androstendione as well as PGE2 have para- and/or autocrine modulatory effects on basal and FSH-stimulated inhibin secretion by mature porcine g-cells in vitro and further demonstrate that the secretion of the proteohormone inhibin and the steroid progesterone are closely related. |
| Databáze: | OpenAIRE |
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