Lysyl oxidase (lox) gene deficiency affects osteoblastic phenotype
| Autor: | Jean-Pierre Bernimoulin, Andrej M. Kielbassa, A. Ding, Joni M. Mäki, Johanna Myllyharju, T. A. L. J. J. Bronckers, P. N’Guessan, Philip C. Trackman, N. Heng, Amitha H. Palamakumbura, P. Weisshaupt, Bernd-Michael Kleber, Ralf J. Radlanski, H. Renz, Nicole Pischon |
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| Přispěvatelé: | Orale Celbiologie (OUD, ACTA) |
| Rok vydání: | 2009 |
| Předmět: |
Bone sialoprotein
musculoskeletal diseases endocrine system diseases Endocrinology Diabetes and Metabolism Down-Regulation Lysyl oxidase Apoptosis Core Binding Factor Alpha 1 Subunit Article Collagen Type I Protein-Lysine 6-Oxidase Mice Endocrinology medicine Animals Orthopedics and Sports Medicine Osteopontin Gene Silencing Mice Knockout Osteoblasts biology integumentary system Skull Wild type Gene Expression Regulation Developmental food and beverages Osteoblast Cell Differentiation DNA Molecular biology RUNX2 Isoenzymes Mice Inbred C57BL enzymes and coenzymes (carbohydrates) medicine.anatomical_structure Phenotype biology.protein Elastin Type I collagen |
| Zdroj: | Calcified Tissue International, 85(2), 119-126. Springer New York Pischon, N, Mäki, J M, Weisshaupt, P, Heng, N, Palamakumbura, A H, N'Guessan, P, Ding, A, Radlanski, R, Renz, H, Bronckers, T A L J J, Myllyharju, J, Kielbassa, A, Kleber, B M, Bernimoulin, J P & Trackman, P C 2009, ' Lysyl oxidase (lox) gene deficiency affects osteoblastic phenotype ', Calcified Tissue International, vol. 85, no. 2, pp. 119-126 . https://doi.org/10.1007/s00223-009-9252-8 |
| ISSN: | 0171-967X |
| Popis: | Lysyl oxidase (LOX) catalyzes cross-linking of elastin and collagen, which is essential for the structural integrity and function of bone tissue. The present study examined the role of Lox gene deficiency for the osteoblast phenotype in primary calvarial osteoblasts from E18.5 Lox knockout (Lox −/− ) and wild type (wt) (C57BL/6) mice. Next to Lox gene depletion, mRNA expression of Lox isoforms, LOXL1–4, was significantly downregulated in Lox −/− bone tissue. A significant decrease of DNA synthesis of Lox −/− osteoblasts compared to wt was found. Early stages of osteoblastic apoptosis studied by annexin-V binding as well as later stages of DNA fragmentation were not affected. However, mineral nodule formation and osteoblastic differentiation were markedly decreased, as revealed by significant downregulation of osteoblastic markers, type I collagen, bone sialoprotein, and Runx2/Cbfa1. |
| Databáze: | OpenAIRE |
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