Peptidomics and proteomics data of oxidised peptides from in vitro gastrointestinal digestion of chicken breast exposed to chlorpyrifos
| Autor: | Fidel Toldrá, Darío Méndez-Cuadro, Erika Rodríguez-Cavallo, Leticia Mora, Johana Márquez-Lázaro |
|---|---|
| Přispěvatelé: | Ministerio de Economía, Industria y Competitividad (España), European Commission |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Proteomics
Protein oxidation medicine.disease_cause lcsh:Computer applications to medicine. Medical informatics chlorpyrifos Chicken breast 03 medical and health sciences 0302 clinical medicine Liquid chromatography–mass spectrometry Agricultural and Biological Science Oxidation medicine lcsh:Science (General) Peptidomics Carcinogen 030304 developmental biology 0303 health sciences Multidisciplinary Chemistry In vitro Biochemistry lcsh:R858-859.7 Digestion Chlorpyrifos 030217 neurology & neurosurgery Genotoxicity Oxidative stress lcsh:Q1-390 |
| Zdroj: | Data in Brief Digital.CSIC. Repositorio Institucional del CSIC instname Data in Brief, Vol 32, Iss, Pp 106160-(2020) |
| ISSN: | 2352-3409 |
| Popis: | To investigate the oxidative effect of CPF on chicken breast proteins, peptidomics and proteomics analysis were used. For this, chicken breast samples were exposed to CPF and subjected to simulated gastrointestinal digestion. The identification of oxidized peptides from digested and undigested proteins were performed by LC MS/MS (nanoESI qQTOF). Prior to mass analyses undigested proteins were trypsinated. Data were analysed using MASCOT and ProteinPilot v 4.5 software. In this study, 90 and 107 oxidized peptides from digested proteins of control and exposed samples were identified, respectively. These peptides corresponding to 12 oxidized proteins. Meanwhile, 260 and 324 oxidized peptides from undigested proteins (control and exposed samples) were identified, which corresponding to 19 and 17 proteins, respectively. Collagen was protein more susceptible to oxidation promoted by chlorpyrifos in digested and undigested proteins. Identification of these oxidized proteins from simulated digestion provides an important insight about the impact of substances like certain veterinary drugs at trace concentrations on the nutritional value of chicken breast meat. Grant AGL2017-89381-R and FEDER funds from the Spanish Ministry of Economy, Industry and Competitiveness, and Ramón y Cajal postdoctoral contract by L.M. are acknowledged. Also COLCIENCIAS and University of Cartagena for financial support (2019 and 2020) to the project Code 1107-711-50102 are acknowledged. |
| Databáze: | OpenAIRE |
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