Adipose Tissue-Specific Deletion of 12/15-Lipoxygenase Protects Mice from the Consequences of a High-Fat Diet
| Autor: | Jerry L. Nadler, Kendall A. Leone, Margaret A. Morris, Wojciech J. Grzesik, Banumathi K. Cole |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Male
medicine.medical_specialty FGF21 Article Subject Adipose tissue macrophages Immunology Adipose tissue Inflammation White adipose tissue Biology Arachidonate 12-Lipoxygenase Diet High-Fat Proinflammatory cytokine Mice 03 medical and health sciences 0302 clinical medicine Insulin resistance Insulin-Secreting Cells Internal medicine lcsh:Pathology medicine Animals Arachidonate 15-Lipoxygenase 030304 developmental biology 0303 health sciences Macrophages Pancreatic islets Cell Biology medicine.disease Lipids Mice Inbred C57BL Glucose Endocrinology medicine.anatomical_structure Adipose Tissue 030220 oncology & carcinogenesis medicine.symptom lcsh:RB1-214 Research Article |
| Zdroj: | Mediators of Inflammation Mediators of Inflammation, Vol 2012 (2012) |
| ISSN: | 1466-1861 0962-9351 |
| Popis: | Type 2 diabetes is associated with obesity, insulin resistance, and inflammation in adipose tissue. 12/15-Lipoxygenase (12/15-LO) generates proinflammatory lipid mediators, which induce inflammation in adipose tissue. Therefore we investigated the role of 12/15-LO activity in mouse white adipose tissue in promoting obesity-induced local and systemic inflammatory consequences. We generated a mouse model for fat-specific deletion of 12/15-LO,aP2-Cre;12/15-LOloxP/loxP, which we call ad-12/15-LO mice, and placed wild-type controls and ad-12/15-LO mice on a high-fat diet for 16 weeks and examined obesity-induced inflammation and insulin resistance. High-fat diet-fed ad-12/15-LO exhibited improved fasting glucose levels and glucose metabolism, and epididymal adipose tissue from these mice exhibited reduced inflammation and macrophage infiltration compared to wild-type mice. Furthermore, fat-specific deletion of 12/15-LO led to decreased peripheral pancreatic islet inflammation with enlarged pancreatic islets when mice were fed the high-fat diet compared to wild-type mice. These results suggest an interesting crosstalk between 12/15-LO expression in adipose tissue and inflammation in pancreatic islets. Therefore, deletion of 12/15-LO in adipose tissue can offer local and systemic protection from obesity-induced consequences, and blocking 12/15-LO activity in adipose tissue may be a novel therapeutic target in the treatment of type 2 diabetes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|