HPLC determination of four triterpenoids in rat urine after oral administration of total triterpenoids from Ganoderma lucidum
| Autor: | Xiaoming Wang, Jiang Hao Sun, Kai Shun Bi, Shu-Hong Guan, Yan Liang, De-An Guo, Wei Wang, Rong Xia Liu, Min Yang |
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| Rok vydání: | 2007 |
| Předmět: |
Quality Control
Magnetic Resonance Spectroscopy Reishi Spectrophotometry Infrared Clinical Biochemistry Pharmaceutical Science Urine Pharmacognosy High-performance liquid chromatography Mass Spectrometry Analytical Chemistry Rats Sprague-Dawley Excretion Triterpene Oral administration Drug Discovery Animals Chromatography High Pressure Liquid Spectroscopy chemistry.chemical_classification Chromatography Chemistry Extraction (chemistry) Reproducibility of Results Reference Standards Triterpenes Rats Calibration Indicators and Reagents Spectrophotometry Ultraviolet Quantitative analysis (chemistry) |
| Zdroj: | Journal of Pharmaceutical and Biomedical Analysis. 43:1185-1190 |
| ISSN: | 0731-7085 |
| DOI: | 10.1016/j.jpba.2006.10.010 |
| Popis: | A sensitive and simple high-performance liquid chromatography (HPLC) method was applied for the quantitative determination of four major triterpenoids (ganoderic acids C 2 , B, K and H) in rat urine after oral administration of total triterpenoids from Ganoderma lucidum . The urine sample was extracted with dichloromethane–ethyl acetate (90:10) after acidification by hydrochloric acid (0.2 mol/ml). Chromatographic separation was achieved on a Zorbax SB-C 18 column (250 mm × 4.6 mm, 5 μm) at 35 °C, with a linear gradient of acetonitrile and 0.03% aqueous phosphoric acid (v/v), at a flow rate of 1.2 ml/min. The four triterpenoids and internal standard (hydrocortisone) were detected at a wavelength 252 nm. The within- and between-day assay coefficients of variation for the four triterpenoids in urine were less than 9% and the extraction recovery of this method was higher than 90%. Using this method, the excretion profile of the triterpenoids in rat urine after oral administration of total triterpenoids of G. lucidum was revealed for the first time. |
| Databáze: | OpenAIRE |
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