The inhibitory effect of betulinic acid on epithelial–mesenchymal transition pathway in renal cell carcinoma

Autor: Arzu Ergen, Bedia Cakmakoglu, Sinem Bireller, MERVE NUR ATAS, Baris Ertugrul
Přispěvatelé: İplik, Elif Sinem
Rok vydání: 2022
Předmět:
Zdroj: Medical Oncology. 39
ISSN: 1559-131X
Popis: Renal cancer is the most lethal urological cancer and characterized by high metastasis rate at initial diagnosis and drug resistance to current chemotherapeutics. Betulinic acid is a pentacyclic triterpene with broad biological activity that occurs naturally in variety of plants. Even though the anti-cancer efficacy of betulinic acid have been reported by many studies, the information about the pathways and the molecules which are affected by betulinic acid in renal cancer are limited. Epithelial-mesenchymal transition (EMT) is considered as the initial step of metastasis and contributes to drug resistance of cancer cells. Depending on the role of EMT in cancer progression and drug resistance, targeting EMT may represent an effective strategy in this context. Therefore, we aimed to investigate the anti-metastatic effects of betulinic acid on renal cell carcinoma cells by evaluating two EMT markers, SNAIL-1, and SDC-2. Following the treatment of betulinic acid at determined doses by WST-1 cytotoxicity assay in our previous study, SDC-2 expression level was decreased in both cell lines. Additionally, in correlation with this result, we also found a reduction in SDC-2 and SNAIL-1 protein levels which are measured by ELISA. Furthermore, the migration and invasion capacities were suppressed by betulinic acid treatment in metastatic renal adenocarcinoma ACHN cells. Taken together, our findings indicate that betulinic acid may constitute a potential treatment approach for renal cancer with further investigations. Istanbul University--TSA-2019-35150 WOS:000841289400014 2-s2.0-85136093961 PMID: 35972672
Databáze: OpenAIRE
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