Drought influences fungal community dynamics in the grapevine rhizosphere and root microbiome
| Autor: | David Gramaje, Matías Gelabert, Dorota Tekielska, Aleš Eichmeier, Rebeca Bujanda, María Julia Carbone, Sandra Alaniz, Pedro Mondino |
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| Přispěvatelé: | Ministry of Education, Youth and Sports (Czech Republic) |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Microbiology (medical)
food.ingredient High-throughput next generation sequencing QH301-705.5 Water stress Bulk soil Plant Science Article food Funneliformis Vitis vinifera L Botany Biology (General) Ecology Evolution Behavior and Systematics Cylindrocarpon Abiotic component Rhizosphere biology Drought fungi Root microbiome Plant physiology food and beverages biology.organism_classification Black-foot disease Rootstock |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname Journal of Fungi, Vol 7, Iss 686, p 686 (2021) RIUR. Repositorio Institucional de la Universidad de La Rioja Journal of Fungi Volume 7 Issue 9 |
| ISSN: | 0101-0018 |
| Popis: | Plant roots support complex microbial communities that can influence nutrition, plant growth, and health. In grapevine, little is known about the impact of abiotic stresses on the below-ground microbiome. In this study, we examined the drought-induced shifts in fungal composition in the root endosphere, the rhizosphere and bulk soil by internal transcribed spacer (ITS) high-throughput amplicon sequencing (HTAS). We imposed three irrigation regimes (100%, 50%, and 25% of the field capacity) to one-year old grapevine rootstock plants cv. SO when plants had developed 2–3 roots. Root endosphere, rhizosphere, and bulk soil samples were collected 6-and 12-months post-plantation. Drought significantly modified the overall fungal composition of all three compartments, with the root endosphere compartment showing the greatest divergence from well-watered control (100%). The overall response of the fungal microbiota associated with black-foot disease (Dactylonectria and “Cylindrocarpon” genera) and the potential biocontrol agent Trichoderma to drought stress was consistent across compartments, namely that their relative abundances were significantly higher at 50–100% than at 25% irrigation regime. We identified a significant enrichment in several fungal genera such as the arbuscular mycorrhizal fungus Funneliformis during drought at 25% watering regime within the roots. Our results reveal that drought stress, in addition to its well-characterized effects on plant physiology, also results in the restructuring of grapevine root microbial communities, and suggest the possibility that members of the altered grapevine microbiota might contribute to plant survival under extreme environmental conditions. This work was partially supported by project No. TP01010018 and by the infrastructure obtained from project CZ.02.1.01/0.0/0.0/16_017/0002334. |
| Databáze: | OpenAIRE |
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