Upregulation of the long non-coding RNA CBR3-AS1 predicts tumor prognosis and contributes to breast cancer progression
| Autor: | Zhiqiang Sun, Yajun Zhu, Yinghua Tang, Jian Wang, Lingyun Xu, Fei Gao, Hong Zhu |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
FACS
fluorescence activated cell sorter 0301 basic medicine MTT 3-(4 5-dimethylthiazol-2-yl)-2 5-diphenyltetrazolium bromide lcsh:QH426-470 Annexin V+/PI+ Annexin V and PI positive stained Biology Malignancy Article law.invention 03 medical and health sciences 0302 clinical medicine Breast cancer lncRNA Downregulation and upregulation law Genetics medicine skin and connective tissue diseases Annexin V+/PI− Annexin V positive and PI negative stained ATCC American Type Culture Collection Cell growth lncRNAs long non-coding RNAs General Medicine medicine.disease Prognosis Tumor progression Long non-coding RNA lcsh:Genetics 030104 developmental biology Apoptosis 030220 oncology & carcinogenesis Cancer research CBR3-AS1 Suppressor |
| Zdroj: | Gene: X, Vol 2, Iss, Pp-(2019) Gene: X |
| ISSN: | 2590-1583 |
| Popis: | Breast cancer is the most common female malignancy and the major cause of cancer-related death in women. Long non-coding RNAs (lncRNAs), as oncogenic or tumor suppressor factor, involved in the development and progression of various cancers. In this study, we sought to investigate the function of lncRNA CBR3-AS1 in breast cancer. We evaluated the expression pattern of CBR3-AS1 in breast cancer tissues and cell lines, explored the correlation between CBR3-AS1 expression and the survival time of breast cancer patients, and probed the effect of CBR3-AS1 on tumor progression of breast cancer through loss-of-function and gain-of-function strategies. Our results showed that CBR3-AS1 was overexpressed in breast cancer tissues and cell lines and predicted the prognosis of breast cancer patients. And CBR3-AS1 exerted biological function as an oncogenic lncRNA, involved in the regulation of cell proliferation, colony formation, apoptosis and tumor growth in breast cancer. Taken together, CBR3-AS1 was up-regulated in breast cancer and promoted the risk of breast cancer. It may be a novel therapeutic target and potential prognostic marker for breast cancer. Highlights • lncRNA CBR3-AS1 is up-regulated in breast cancer tissues and cell lines. • High CBR3-AS1 expression predicted poor prognosis in breast cancer patients. • CBR3-AS1 promote breast cancer cell growth in vitro and in vivo. |
| Databáze: | OpenAIRE |
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