Ocular Pharmacokinetics of 25-Hydroxyvitamin D3 After Weekly Supplementation in Rabbits Using Ultra Performance Liquid Chromatography-Tandem Mass Spectrometer
| Autor: | Archit Kumar, Tripti Rungta, Ajay Patial, Savita Verma Attri, Radha Kanta Ratho, Bikash Medhi, Mini P Singh |
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| Rok vydání: | 2018 |
| Předmět: |
Clinical chemistry
Administration Oral 030209 endocrinology & metabolism Mass spectrometry Drug Administration Schedule Aqueous Humor 03 medical and health sciences 0302 clinical medicine Tandem Mass Spectrometry Paracentesis medicine Vitamin D and neurology Animals Pharmacology (medical) Deuterium labeled Chromatography High Pressure Liquid Calcifediol Pharmacology Chromatography medicine.diagnostic_test Vitamin d supplementation business.industry eye diseases Ocular pharmacokinetics Intraocular fluid Dietary Supplements 030221 ophthalmology & optometry sense organs Rabbits business |
| Zdroj: | European journal of drug metabolism and pharmacokinetics. 43(5) |
| ISSN: | 2107-0180 |
| Popis: | The protective role of vitamin D supplementation has recently been shown to be present in various ocular inflammatory diseases. The oral supplementation of vitamin D may take time to achieve adequate levels in intraocular fluids. Therefore, the present study was performed to understand the ocular pharmacokinetics of 25-hydroxyvitamin D3 (25D3) in aqueous humor after weekly supplementation of 25D3 in rabbits. A total of 21 rabbits were fed orally with 25D3 (7.22 µg/kg/week) for 8 weeks and 9th dose was given at the end of 8 weeks. The blood and aqueous humor samples were collected from ear vein and though anterior chamber paracentesis, respectively. The serum and aqueous humor samples were spiked with deuterium labeled internal standard and were extracted using liquid extraction method. Furthermore, the samples were derivatized and 25D3 estimation was performed using ultra performance liquid chromatography–tandem mass spectrometer (UHPLC–MS/MS). The 25D3 supplementation significantly increased the 25D3 levels in serum (78.5 ± 21.6 ng/ml) (mean ± SD) (p |
| Databáze: | OpenAIRE |
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