Developmental Origin of a Bipotential Myocardial and Smooth Muscle Cell Precursor in the Mammalian Heart

Autor: Susan M. Cibulsky, Yuko Fujiwara, Thomas M. Schultheiss, Stuart H. Orkin, David E. Clapham, Sean M. Wu, Ching-Ling Lien
Rok vydání: 2006
Předmět:
Mesoderm
Cellular differentiation
Green Fluorescent Proteins
Myocytes
Smooth Muscle
Cell
Population
Mice
Transgenic
Cell Separation
Biology
Muscle
Smooth
Vascular
General Biochemistry
Genetics and Molecular Biology
Mice
03 medical and health sciences
0302 clinical medicine
medicine
Animals
Myocyte
Cell Lineage
Myocytes
Cardiac
education
Embryonic Stem Cells
030304 developmental biology
Homeodomain Proteins
0303 health sciences
education.field_of_study
Heart development
Biochemistry
Genetics and Molecular Biology(all)
Multipotent Stem Cells
Myocardium
Gene Expression Regulation
Developmental
Cell Differentiation
Heart
Embryonic stem cell
Cell biology
Mice
Inbred C57BL
Proto-Oncogene Proteins c-kit
medicine.anatomical_structure
Multipotent Stem Cell
Homeobox Protein Nkx-2.5
030217 neurology & neurosurgery
Transcription Factors
Zdroj: Cell. 127:1137-1150
ISSN: 0092-8674
Popis: Despite recent advances in delineating the mechanisms involved in cardiogenesis, cellular lineage specification remains incompletely understood. To explore the relationship between developmental fate and potential, we isolated a cardiac-specific Nkx2.5(+) cell population from the developing mouse embryo. The majority of these cells differentiated into cardiomyocytes and conduction system cells. Some, surprisingly, adopted a smooth muscle fate. To address the clonal origin of these lineages, we isolated Nkx2.5(+) cells from in vitro differentiated murine embryonic stem cells and found approximately 28% of these cells expressed c-kit. These c-kit(+) cells possessed the capacity for long-term in vitro expansion and differentiation into both cardiomyocytes and smooth muscle cells from a single cell. We confirmed these findings by isolating c-kit(+)Nkx2.5(+) cells from mouse embryos and demonstrated their capacity for bipotential differentiation in vivo. Taken together, these results support the existence of a common precursor for cardiovascular lineages in the mammalian heart.
Databáze: OpenAIRE
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