Inheritance Pattern of RAPD Markers in Melipona quadrifasciata (Hymenoptera: Apidae, Meliponinae)
| Autor: | E. G. Barros, L. A. O. Campos, M. G. Tavares, E. H. Ribeiro, M. T. V. A. Oliveira |
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| Rok vydání: | 2001 |
| Předmět: |
Genetic Markers
Biology Polymerase Chain Reaction law.invention Hydroxybutyrate Dehydrogenase symbols.namesake Polymorphism (computer science) law Genetics Animals Molecular Biology Genetics (clinical) Polymerase chain reaction Genomic organization Polymorphism Genetic Bees DNA Protozoan Random Amplified Polymorphic DNA Technique RAPD genomic DNA Genetic marker Mendelian inheritance symbols Genome Protozoan Biotechnology Melipona quadrifasciata |
| Zdroj: | Journal of Heredity. 92:279-282 |
| ISSN: | 1465-7333 0022-1503 |
| DOI: | 10.1093/jhered/92.3.279 |
| Popis: | Melipona quadrifasciata is an important pollinator agent in several regions of Brazil. Data concerning the genetics of this species are scarce in the literature. In this work we used the random amplified polymorphic DNA (RAPD) technique to determine the degree of polymorphism and the inheritance pattern of these molecular markers in this species. Our ultimate goal is to establish tools to be used in the study of the genomic organization of M. quadrifasciata. Genomic DNA from progenies F(1) and BC(1) were assayed with 79 different primers, yielding an average of 6.67 bands and 1.68 polymorphisms per primer. Three types of polymorphisms were detected: band presence/absence, band intensity, and fragment-length polymorphisms. Most of the observed polymorphisms were band presence/absence, typical of RAPD-dominant markers. The number of observed polymorphisms and their segregation in accordance with a Mendelian proportion confirm the importance of this technique for genome analysis of species like M. quadrifasciata that are poorly studied at the genetic level. |
| Databáze: | OpenAIRE |
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