Labelling of bleomycin with Auger-emitter increases cytotoxicity in squamous-cell cancer cell lines
| Autor: | Hans Ramsay, H. A. Jaaskela-Saari, Kalevi Kairemo, Reidar Grénman |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
congenital
hereditary and neonatal diseases and abnormalities medicine.medical_specialty Antimetabolites Antineoplastic Bleomycin Indium chemistry.chemical_compound Labelling medicine Tumor Cells Cultured Humans Radiology Nuclear Medicine and imaging Cytotoxicity Clonogenic assay Anticarcinogen Radiological and Ultrasound Technology Chemistry Indium Radioisotopes nutritional and metabolic diseases Molecular biology Combined Modality Therapy Surgery Cell killing Epidermoid carcinoma Cell culture Head and Neck Neoplasms Isotope Labeling Carcinoma Squamous Cell Drug Screening Assays Antitumor |
| Zdroj: | International journal of radiation biology. 73(5) |
| ISSN: | 0955-3002 |
| Popis: | Purpose: To investigate the cytotoxicity of bleomycin (BLM), two Auger-emitting bleomycin complexes (indium-111 ( 111 In)-BLMC) and 111 InCl 3 in three squamous cell cancer (SCC) cell lines. Material and methods: Three recently established SCC cell lines were investigated using the 96-well clonogenic assay. Concentrations causing 50% inhibition in cell survival (IC 50 ) were calculated for BLM and two specific activities of 111 In-BLMC (40 MBq/mg BLM (low) and 195 MBq/mg BLM (high)). Results: 111 In-BLMC (low) was the most toxic to the SCC cell lines. 111 In-BLMC containing 4.9-fold more activity of 'In (195 MBq/mg BLM) was more effective than BLM (p = 0.0029), but not as toxic as 111 In-BLMC (low) (p=0.0023). UT-SCC-19A had a IC 50 value for BLM as low as 4.1 nM, whereas IC 50 values for 111 In-BLMC (low) and 111 In-BLMC (high) were 2.0 nM and 2.6 nM, respectively. The most chemoresistant cell line UT-SCC-12A had a IC 50 value for BLM of 18.8 nM, for 111 In-BLMC (low) 10.7 nM and for 111 In-BLMC (high) 12.7 nM. 111 InCl 3 had no cell killing effect. Conclusions: This study shows that 111 In-BLMC is superior in SCC cell killing compared with BLM. These data provide the basis for further clinical investigations of 111 In-BLMC. |
| Databáze: | OpenAIRE |
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