Trophoblastic invasion in vitro and in vivo: similarities and differences
| Autor: | Astrid Hammer, Berthold Huppertz, Gottfried Dohr, C. Helige, Helmut Ahammer, H.-G. Frank |
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| Rok vydání: | 2008 |
| Předmět: |
Apoptosis
Biology Cell Line Natural killer cell HLA Antigens Pregnancy In vivo Spheroids Cellular Decidua Leukocytes medicine Humans reproductive and urinary physiology Cell Proliferation HLA-G Antigens Histocompatibility Antigens Class I Rehabilitation Choriocarcinoma Obstetrics and Gynecology Placentation Trophoblast medicine.disease Coculture Techniques In vitro Trophoblasts Cell biology medicine.anatomical_structure Reproductive Medicine Cell culture embryonic structures Immunology Natural Killer T-Cells Female |
| Zdroj: | Human Reproduction. 23:2282-2291 |
| ISSN: | 1460-2350 0268-1161 |
| DOI: | 10.1093/humrep/den198 |
| Popis: | BACKGROUND: The basic mechanisms of trophoblast invasion are not completely understood. This may be due to the lack of suitable in vitro models which enable experimental modulation of this complex process. In the present study, a three-dimensional co-culture model is used for comparing two factors considered to be implicated in the regulation of trophoblast invasion, the expression of HLA-G and apoptosis, in vitro and in vivo. METHODS: Tissue fragments from human first trimester decidua parietalis were put in close contact with spheroids of AC-1M59 trophoblast/choriocarcinoma hybrid cells as a model of the invasive trophoblast. Cryostat sections from these co-cultures were immunohistochemically stained and compared with first trimester placentation sites in vivo. RESULTS: Only the invasive trophoblast-derived cells showed an intensive staining for HLA-G, whereas the cells on the periphery of the confrontation culture exhibited only a weak staining. A similar staining pattern was found in vivo. Both in vitro andin vivoCD45 1 apoptotic leukocytes were frequently detected in close proximity to the invasive trophoblastic cells. CONCLUSIONS: In this co-culture system, key factors considered to be implicated in trophoblast invasion in vivo can also be demonstrated in vitro. Therefore, it may help in finding strategies for the management of diseases associated with deficient trophoblast invasion. |
| Databáze: | OpenAIRE |
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