Functional annotation of human long noncoding RNAs via molecular phenotyping

Autor:	Jayson Harshbarger, Hideya Kawaji, Diego Garrido, Michiel J. L. de Hoon, Tsugumi Kawashima, Lusy Handoko, Masayoshi Itoh, John F. Ouyang, Michihira Tagami, Kosuke Hashimoto, Andreas Petri, Patrizia Rizzu, Christopher J. F. Cameron, Tetsuro Hirose, Marina Lizio, Beatrice Borsari, Robert Young, Vidisha Tripathi, Chung-Chau Hon, Joachim Luginbühl, Ryan Cardenas, Jasmine Li Ching Ooi, Chikashi Terao, Vsevolod J. Makeev, Aki Minoda, Colin A. Semple, Alexander V. Favorov, Yasushi Okazaki, Kazuhiro R. Nitta, Mitsuyoshi Murata, Juha Kere, Harukazu Suzuki, Bogumil Kaczkowski, Fernando López-Redondo, Ivan Antonov, Mickaël Mendez, Diego Fernando Sánchez Martinez, Michael M. Hoffman, Chi Wai Yip, Imad Abugessaisa, Pillay Sanjana, Sakari Kauppinen, Erik Arner, Denis Paquette, Norihito Hayatsu, Ramil N. Nurtdinov, Mariola Kurowska-Stolarska, Luigi Marchionni, Takahiro Suzuki, Claudio Schneider, J Kenneth Baillie, Andrew T. Kwon, Saumya Agrawal, Carlo Vittorio Cannistraci, Roberto Verardo, Suzannah C. Szumowski, Frank Brombacher, Tsukasa Kouno, Boris Lenhard, Noa Gil, Manuel Muñoz-Aguirre, Shiori Maeda, Luca Ducoli, Emily Kawabata, Valerio Orlando, Leonie Roos, Divya M. Sivaraman, Youtaro Shibayama, Supat Thongjuea, Piero Carninci, Kayoko Yasuzawa, Jeffrey T. Leek, Alexandre Fort, Hidemasa Bono, Peter Heutink, Takeya Kasukawa, Alessandro Bonetti, Jen-Chien Chang, Josée Dostie, Naoto Kondo, Ferenc Müller, Nicholas J. Parkinson, Haruhiko Koseki, Malte Thodberg, Callum J.C. Parr, Anton Kratz, Miki Kojima, Reto Guler, Jordan A. Ramilowski, Alistair R. R. Forrest, Owen J. L. Rackham, Igor Ulitsky, Yari Ciani, Howard Y. Chang, Roderic Guigó, Jay W. Shin, Andreas Lennartsson, Ivan V. Kulakovskiy, Jessica Severin, Ilya E. Vorontsov, Melissa Cardon, Ken Yagi, Chinatsu Yamamoto, Yukihiko Noro, Juliette Gimenez, Shuhei Noguchi, Yuki Hasegawa, Eddie Luidy Imada, Martin S. Taylor, Yulia A. Medvedeva, Altuna Akalin, Albin Sandelin, Aditi Kanhere, S. Thomas Kelly, Hiromi Nishiyori, Akira Hasegawa
Přispěvatelé:	Wellcome Trust, STEMM - Stem Cells and Metabolism Research Program, Juha Kere / Principal Investigator, Research Programs Unit, University of Helsinki, HUS Helsinki and Uusimaa Hospital District
Jazyk:	angličtina
Rok vydání:	2020
Předmět:	Genome Transcriptome 0302 clinical medicine Cell Movement Transcription (biology) antagonists & inhibitors [RNA Long Noncoding] Gene expression cytology [Fibroblasts] TRANSCRIPTION RNA Small Interfering Genetics (clinical) 11 Medical and Health Sciences GENE-EXPRESSION Genetics & Heredity 0303 health sciences Gene knockdown 318 Medical biotechnology KCNQ Potassium Channels 1184 Genetics developmental biology physiology physiology [RNA Long Noncoding] Phenotype DIFFERENTIATION ddc:540 RNA Long Noncoding Technology Platforms Life Sciences & Biomedicine metabolism [Fibroblasts] Resource Biochemistry & Molecular Biology Bioinformatics UNIQUE FEATURES Cell Growth Processes Computational biology Biology ADULT HUMAN FIBROBLASTS 03 medical and health sciences REVEALS Genetics genetics [Cell Growth Processes] Humans Gene 030304 developmental biology REGULATORS Science & Technology metabolism [KCNQ Potassium Channels] Cell growth genetics [Cell Movement] Molecular Sequence Annotation Fibroblasts Oligonucleotides Antisense 06 Biological Sciences Biotechnology & Applied Microbiology Cardiovascular and Metabolic Diseases PRINCIPLES CELLS metabolism [RNA Long Noncoding] 1182 Biochemistry cell and molecular biology 3111 Biomedicine TRANSLATION 030217 neurology & neurosurgery
Zdroj:	Ramilowski, J A, Yip, C W, Agrawal, S, Chang, J-C, Ciani, Y, Kulakovskiy, I V, Mendez, M, Ooi, J L C, Ouyang, J F, Parkinson, N, Petri, A, Roos, L, Severin, J, Yasuzawa, K, Abugessaisa, I, Akalin, A, Antonov, I V, Arner, E, Bonetti, A, Bono, H, Borsari, B, Brombacher, F, Cameron, C JF, Cannistraci, C V, Cardenas, R, Cardon, M, Chang, H, Dostie, J, Ducoli, L, Favorov, A, Fort, A, Garrido, D, Gil, N, Gimenez, J, Guler, R, Handoko, L, Harshbarger, J, Hasegawa, A, Hasegawa, Y, Hashimoto, K, Hayatsu, N, Heutink, P, Hirose, T, Imada, E L, Itoh, M, Kaczkowski, B, Kanhere, A, Kawabata, E, Kawaji, H, Kawashima, T, Kelly, S T, Kojima, M, Kondo, N, Koseki, H, Kouno, T, Kratz, A, Kurowska-Stolarska, M, Kwon, A T J, Leek, J, Lennartsson, A, Lizio, M, López-Redondo, F, Luginbühl, J, Maeda, S, Makeev, V J, Marchionni, L, Medvedeva, Y A, Minoda, A, Müller, F, Muñoz-Aguirre, M, Murata, M, Nishiyori, H, Nitta, K R, Noguchi, S, Noro, Y, Nurtdinov, R, Okazaki, Y, Orlando, V, Paquette, D, Parr, C J C, Rackham, O J L, Rizzu, P, Sánchez Martinez, D F, Sandelin, A, Sanjana, P, Semple, C A M, Shibayama, Y, Sivaraman, D M, Suzuki, T, Szumowski, S C, Tagami, M, Taylor, M S, Terao, C, Thodberg, M, Thongjuea, S, Tripathi, V, Ulitsky, I, Verardo, R, Vorontsov, I E, Yamamoto, C, Young, R S, Baillie, J K, Forrest, A R R, Guigó, R, Hoffman, M M, Hon, C C, Kasukawa, T, Kauppinen, S, Kere, J, Lenhard, B, Schneider, C, Suzuki, H, Yagi, K, de Hoon, M J L, Shin, J W & Carninci, P 2020, ' Functional annotation of human long noncoding RNAs via molecular phenotyping ', Genome Research, vol. 30, no. 7, pp. 1060-1072 . https://doi.org/10.1101/gr.254219.119 Genome Research Ramilowski, J A, Yip, C W, Agrawal, S, Chang, J-C, Ciani, Y, Kulakovskiy, I V, Mendez, M, Ooi, J L C, Ouyang, J F, Parkinson, N, Petri, A, Roos, L, Severin, J, Yasuzawa, K, Abugessaisa, I, Akalin, A, Antonov, I V, Arner, E, Bonetti, A, Bono, H, Borsari, B, Brombacher, F, Cameron, C J F, Cannistraci, C V, Cardenas, R, Cardon, M, Chang, H, Dostie, J, Ducoli, L, Favorov, A, Fort, A, Garrido, D, Gil, N, Gimenez, J, Guler, R, Handoko, L, Harshbarger, J, Hasegawa, A, Hasegawa, Y, Hashimoto, K, Hayatsu, N, Heutink, P, Hirose, T, Imada, E L, Itoh, M, Kaczkowski, B, Kanhere, A, Kawabata, E, Kawaji, H, Kawashima, T, Kelly, S T, Kojima, M, Kondo, N, Koseki, H, Kouno, T, Kratz, A, Kurowska-Stolarska, M, Kwon, A T J, Leek, J, Lennartsson, A, Lizio, M, López-Redondo, F, Luginbühl, J, Maeda, S, Makeev, V J, Marchionni, L, Medvedeva, Y A, Minoda, A, Müller, F, Muñoz-Aguirre, M, Murata, M, Nishiyori, H, Nitta, K R, Noguchi, S, Noro, Y, Nurtdinov, R, Okazaki, Y, Orlando, V, Paquette, D, Parr, C J C, Rackham, O J L, Rizzu, P, Sánchez Martinez, D F, Sandelin, A, Sanjana, P, Semple, C A M, Shibayama, Y, Sivaraman, D M, Suzuki, T, Szumowski, S C, Tagami, M, Taylor, M S, Terao, C, Thodberg, M, Thongjuea, S, Tripathi, V, Ulitsky, I, Verardo, R, Vorontsov, I E, Yamamoto, C, Young, R S, Baillie, J K, Forrest, A R R, Guigó, R, Hoffman, M M, Hon, C C, Kasukawa, T, Kauppinen, S, Kere, J, Lenhard, B, Schneider, C, Suzuki, H, Yagi, K, de Hoon, M J L, Shin, J W & Carninci, P 2020, ' Functional annotation of human long noncoding RNAs via molecular phenotyping ', Genome Research, vol. 30, pp. 1060-1072 . https://doi.org/10.1101/gr.254219.119 Ramilowski, J A, Yip, C W, Agrawal, S, Chang, J-C, Ciani, Y, Kulakovskiy, I V, Mendez, M, Ooi, J L C, Ouyang, J F, Parkinson, N, Petri, A, Roos, L, Severin, J, Yasuzawa, K, Abugessaisa, I, Akalin, A, Antonov, I V, Arner, E, Bonetti, A, Bono, H, Borsari, B, Brombacher, F, Cameron, C J F, Cannistraci, C V, Cardenas, R, Cardon, M, Chang, H, Dostie, J, Ducoli, L, Favorov, A, Fort, A, Garrido, D, Gil, N, Gimenez, J, Guler, R, Handoko, L, Harshbarger, J, Hasegawa, A, Hasegawa, Y, Hashimoto, K, Hayatsu, N, Heutink, P, Hirose, T, Imada, E L, Itoh, M, Kaczkowski, B, Kanhere, A, Kawabata, E, Kawaji, H, Kawashima, T, Kelly, S T, Kojima, M, Kondo, N, Koseki, H, Kouno, T, Kratz, A, Kurowska-Stolarska, M, Kwon, A T J, Leek, J, Lennartsson, A, Lizio, M, López-Redondo, F, Luginbühl, J, Maeda, S, Makeev, V J, Marchionni, L, Medvedeva, Y A, Minoda, A, Müller, F, Muñoz-Aguirre, M, Murata, M, Nishiyori, H, Nitta, K R, Noguchi, S, Noro, Y, Nurtdinov, R, Okazaki, Y, Orlando, V, Paquette, D, Parr, C J C, Rackham, O J L, Rizzu, P, Sánchez Martinez, D F, Sandelin, A, Sanjana, P, Semple, C A M, Shibayama, Y, Sivaraman, D M, Suzuki, T, Szumowski, S C, Tagami, M, Taylor, M S, Terao, C, Thodberg, M, Thongjuea, S, Tripathi, V, Ulitsky, I, Verardo, R, Vorontsov, I E, Yamamoto, C, Young, R S, Baillie, J K, Forrest, A R R, Guigó, R, Hoffman, M M, Hon, C C, Kasukawa, T, Kauppinen, S, Kere, J, Lenhard, B, Schneider, C, Suzuki, H, Yagi, K, de Hoon, M J L, Shin, J W & Carninci, P 2020, ' Functional annotation of human long noncoding RNAs via molecular phenotyping ', Genome Research . https://doi.org/10.1101/gr.254219.119 Ramilowski, J A, Yip, C W, Agrawal, S, Chang, J-C, Ciani, Y, Kulakovskiy, I V, Mendez, M, Ooi, J L C, Ouyang, J F, Parkinson, N, Petri, A, Roos, L, Severin, J, Yasuzawa, K, Abugessaisa, I, Akalin, A, Antonov, I V, Arner, E, Bonetti, A, Bono, H, Borsari, B, Brombacher, F, Cameron, C J F, Cannistraci, C V, Cardenas, R, Cardon, M, Chang, H, Dostie, J, Ducoli, L, Favorov, A, Fort, A, Garrido, D, Gil, N, Gimenez, J, Guler, R, Handoko, L, Harshbarger, J, Hasegawa, A, Hasegawa, Y, Hashimoto, K, Hayatsu, N, Heutink, P, Hirose, T, Imada, E L, Itoh, M, Kaczkowski, B, Kanhere, A, Kawabata, E, Kawaji, H, Kawashima, T, Kelly, S T, Kojima, M, Kondo, N, Koseki, H, Kouno, T, Kratz, A, Kurowska-Stolarska, M, Kwon, A T J, Leek, J, Lennartsson, A, Lizio, M, Lopez-Redondo, F, Luginbühl, J, Maeda, S, Makeev, V J, Marchionni, L, Medvedeva, Y A, Minoda, A, Müller, F, Muñoz-Aguirre, M, Murata, M, Nishiyori, H, Nitta, K R, Noguchi, S, Noro, Y, Nurtdinov, R, Okazaki, Y, Orlando, V, Paquette, D, Parr, C J C, Rackham, O J L, Rizzu, P, Martinez, D F S, Sandelin, A, Sanjana, P, Semple, C A M, Shibayama, Y, Sivaraman, D M, Suzuki, T, Szumowski, S C, Tagami, M, Taylor, M S, Terao, C, Thodberg, M, Thongjuea, S, Tripathi, V, Ulitsky, I, Verardo, R, Vorontsov, I E, Yamamoto, C, Young, R S, Baillie, J K, Forrest, A R R, Guigó, R, Hoffman, M M, Hon, C C, Kasukawa, T, Kauppinen, S, Kere, J, Lenhard, B, Schneider, C, Suzuki, H, Yagi, K, de Hoon, M J L, Shin, J W & Carninci, P 2020, ' Functional annotation of human long noncoding RNAs via molecular phenotyping ', Genome Research, vol. 30, no. 7, pp. 1060-1072 . https://doi.org/10.1101/gr.254219.119 Genome Res Genome research 30(7), 1060-1072 (2020). doi:10.1101/gr.254219.119
DOI:	10.1101/gr.254219.119
Popis:	Long noncoding RNAs (lncRNAs) constitute the majority of transcripts in the mammalian genomes, and yet, their functions remain largely unknown. As part of the FANTOM6 project, we systematically knocked down the expression of 285 lncRNAs in human dermal fibroblasts and quantified cellular growth, morphological changes, and transcriptomic responses using Capped Analysis of Gene Expression (CAGE). Antisense oligonucleotides targeting the same lncRNAs exhibited global concordance, and the molecular phenotype, measured by CAGE, recapitulated the observed cellular phenotypes while providing additional insights on the affected genes and pathways. Here, we disseminate the largest-to-date lncRNA knockdown data set with molecular phenotyping (over 1000 CAGE deep-sequencing libraries) for further exploration and highlight functional roles for ZNF213-AS1 and lnc-KHDC3L-2.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9cf2df2c4fb7df1b0612e3c14c9242a6 https://vbn.aau.dk/da/publications/9945d8a2-24bb-4411-ada7-c0664ae247f1 Zobrazit plný text záznamu